רÀûÃû³Æ£º»ÆÜοÅÁ£Ö¸ÎÆÍ¼Æ×µÄ²â¶¨·½·¨¼°ÆäÌØÕ÷Ö¸ÎÆÍ¼Æ×µÄÖÆ×÷·½·¨
¼¼ÊõÁìÓò£º
±¾·¢Ã÷Éæ¼°ÖÐÒ©Ö¸ÎÆÍ¼Æ×·ÖÎö·½·¨£¬ÌرðÊÇ»ÆÜοÅÁ£HPLCÌØÕ÷Ö¸ÎÆÍ¼Æ×µÄ²â¶¨·½·¨ÒÔ¼°ÓÉ´Ë·½·¨ËùµÃµ½µÄ»ÆÜοÅÁ£µÄÌØÕ÷Ö¸ÎÆÍ¼Æ×¡£
±³¾°¼¼Êõ£º
ÎÒ¹ú´«Í³µÄÖÐÒ©¼°ÆäÖÆ¼Á´ó¶àȱ·¦ÑÏÃܵÄÖÊÁ¿±ê×¼ºÍ¿ÆÑ§µÄ¼ì²âÊֶΣ¬ÄÑÒÔÓÐЧµÄ¿ØÖÆÆäÄÚÔÚÖÊÁ¿£¬²»Äܱ£Ö¤ÓÃÒ©µÄ°²È«¡¢ÓÐЧ£¬Ò²²»·ûºÏ¹ú¼ÊÒ½Ò©Êг¡µÄÒªÇó£¬ÑÏÖØÖÆÔ¼ÁËÎÒ¹úÖÐÒ©ÐÐÒµµÄ·¢Õ¹¡£¼ÓÇ¿ÖÐÒ©²ÄÖÊÁ¿¿ØÖÆÑо¿ÊÇÖÐÒ©¹æ·¶»¯¡¢±ê×¼»¯µÄ¹Ø¼üÎÊÌâ¡£Ö»ÓжÔÖÐÒ©²Ä½øÐпÆÑ§µÄÖÊÁ¿¿ØÖÆ£¬²ÅÄܱ£Ö¤ÖÐÒ©ÖÊÁ¿£¬ÊµÏÖÖÐÒ©µÄ¡°°²È«¡¢ÓÐЧ¡¢Îȶ¨¡¢¿É¿Ø¡±¡£»ÆÜοÅÁ£ÊÕÔØÓÚ¡¶ÖÐÒ©²¿°ä±ê×¼¡·£¬±ê×¼±àºÅ:WS3-B-2224_96£¬ÊÇ»ÆÜÎÒ©²ÄÈ«³É·ÖÌáÈ¡Îï£¬Îª×Ø»ÆÉ«¿ÅÁ££¬¸ÃÒ©ÁÆÐ§È·ÇУ¬ÁÙ´²Ó¦Óù㷺£¬¶ÔÂýÐÔÉö²¡¡¢ÐÄѪ¹Üϵͳ¼²²¡¡¢ÌÇÄò²¡¡¢Ö×Áö»¯ÁÆ·ÅÁÆÒÔ¼°ÊÖÊõºó¡¢ÂýÐÔ±ÇÑס¢¹ÇÖÊÊèËɵȼ²²¡¾ßÓнϺõÄÁÙ´²Ð§¹û¡£¸Ã±ê×¼ÖÐÖ÷ÒªÒÔ±¡²ãÉ«Æ×¶¨ÐÔ¼ø±ð·½·¨¿ØÖÆÖƼÁÖÊÁ¿£¬Ä¿Ç°Êг¡ÉÏÉú²ú»ÆÜοÅÁ£µÄ³§¼Ò½Ï¶à£¬ÁÙ´²ÐèÇóÁ¿¾Þ´ó£¬ÏÖÐеÄÖÊÁ¿¿ØÖƼ¼Êõ±ê×¼ÒѾ²»ÄÜÂú×ã»ÆÜοÅÁ£Ó¦Ó÷¢Õ¹Ö®ÐèÒª¡£½üÄêÀ´£¬¹ØÓÚ»ÆÜοÅÁ£ÖÊÁ¿¿ØÖÆÒÑÓÐÒ»¶¨Ñо¿±¨µÀ£¬ÓÐѧÕß²ÉÓ÷ֹâ¹â¶È·¨½¨Á¢»ÆÜοÅÁ£ÖÐ×Ü»ÆÍªµÄº¬Á¿²â¶¨·½·¨£¬ËÄ´¨´óѧ»ªÎ÷ҩѧԺ¶Ô»ÆÜοÅÁ£ÖлÆÜμ×ÜÕºÍ×ÜÔíÜÕº¬Á¿²â¶¨½øÐÐÁËÑо¿£¬ÕâЩÑо¿Îª»ÆÜοÅÁ£µÄÖÊÁ¿¿ØÖÆÌṩÁËÒ»¶¨²Î¿¼£¬¾ßÓÐÒ»¶¨¼ÛÖµ¡£Ä¿Ç°£¬Ò²ÓÐһЩ²ÉÓÃHPLC½¨Á¢»ÆÜοÅÁ£µÄÌØÕ÷Ö¸ÎÆÍ¼Æ×·ÖÎö·½·¨£¬±ÈÈç:¹«¿ªºÅΪ¡°CN102353729A¡±¡¢Ãû³ÆÎª¡°Ò»ÖÖ»ÆÜÎÒ©²ÄµÄÖÊÁ¿¼ì²â·½·¨¡±µÄרÀûÎÄÏ×ÖУ¬²ÉÓÃÒÒëæ-ˮϵͳ×÷ΪÁ÷¶¯Ïàϵͳ£¬µ«µÃµ½µÄÖ¸ÎÆÍ¼Æ×ÖÐÖ»ÓÐ7¸ö¹²ÓÐÉ«Æ×·å£»²¢ÇÒÆäÕë¶ÔµÄÊÇ»ÆÜÎÒ©²Ä£¬¶ø²»ÊÇ»ÆÜοÅÁ£ÖƼÁ¡£¹«¸æºÅΪ¡°CN101327246B¡±¡¢Ãû³ÆÎª¡°»ÆÜÎÒ©²Ä¡¢ÖмäÌå¼°ÆäÖÆ¼ÁµÄÖ¸ÎÆÍ¼Æ×¼ì²â·½·¨ºÍ±ê×¼Ö¸ÎÆÍ¼Æ×¡±µÄרÀûÎļþÖУ¬²ÉÓÃ80%-95%µÄ¼×´¼×÷ΪÌáÈ¡ÈܼÁ£¬ÒÒëæ-ˮϵͳ×÷ΪÁ÷¶¯Ïàϵͳ£¬ÆäµÃµ½µÄÖ¸ÎÆÍ¼Æ×·ÖÀë¶È²»ÊǺܺã¬Ö»ÓÐ19¸öÌØÕ÷É«Æ×·å¡£
·¢Ã÷ÄÚÈÝ
±¾·¢Ã÷µÄÄ¿µÄÔÚÓÚ:ÌṩһÖÖ»ÆÜοÅÁ£Ö¸ÎÆÍ¼Æ×µÄ²â¶¨·½·¨¼°ÆäÌØÕ÷Ö¸ÎÆÍ¼Æ×¡£±¾·¢Ã÷ͨ¹ý¶Ô»ÆÜοÅÁ£HPLCÖ¸ÎÆÍ¼Æ×µÄÑо¿£¬ÌṩÁËÒ»ÖÖ¿ìËÙÆÀ¼Û»ÆÜοÅÁ£ÖÊÁ¿µÄ·½·¨£¬ÃÖ²¹ÁËÏÖÓÐÖÊÁ¿¿ØÖƼ¼ÊõµÄ²»×㣬ʹ»ÆÜοÅÁ£µÄÖÊÁ¿¿ØÖƼ¼Êõ¸üΪÍêÉÆ¡¢¿ÆÑ§¡£±¾·¢Ã÷µÄ¼¼Êõ·½°¸:»ÆÜοÅÁ£Ö¸ÎÆÍ¼Æ×µÄ²â¶¨·½·¨°üÀ¨ÒÔϲ½Öè:( I)¹©ÊÔÆ·ÈÜÒºµÄÖÆ±¸:È¡»ÆÜοÅÁ£·ÛË飬¹ýɸ£¬³ÆÈ¡·ÛÄ©£¬¼Ó¼×´¼ÈÜÒºÈܽ⣬³¬ÉùÌáÈ¡I 2´Î£¬Ã¿´Î5 30min£¬È»ºóÀäÈ´£¬²¹×ãËðÊ§ÖØÁ¿£¬Ò¡ÔÈ£¬Â˹ý£¬È¡ÐøÂËÒº£¬¼´µÃ¹©ÊÔÆ·ÈÜÒº£»(2)¶ÔÕÕÆ·ÈÜÒºµÄÖÆ±¸:È¡¼õѹ¸ÉÔïÖÁºãÖØµÄëÈïÒì»ÆÍªÜÕ¡¢Ã¢±ú»¨ÜÕ¡¢Ã«ÈïÒì»ÆÍª¡¢Ã¢±ú»¨ËضÔÕÕÆ·£¬·Ö±ð¼ÓÈë¼×´¼ÖƳɶÔÕÕÆ·ÈÜÒº£»(3)²â¶¨:·Ö±ð¾«ÃÜÎüÈ¡¹©ÊÔÆ·ÈÜÒººÍ¶ÔÕÕÆ·ÈÜÒº£¬×¢Èë¸ßЧҺÏàÉ«Æ×ÒDzⶨ£¬¼Ç¼ɫÆ×ͼ£»¹©ÊÔÆ·ÈÜҺעÈë¸ßЧҺÏàÉ«Æ×ÒǺó£¬ÒÔ¼×´¼-0.5%¼×ËáˮΪÁ÷¶¯Ïà½øÐÐÌݶÈÏ´ÍÑ£»(4)ÓÃÖ¸ÎÆÍ¼Æ×Èí¼þ¶ÔËùµÃµÄͼÆ×½øÐд¦Àí£¬¼´µÃµ½»ÆÜοÅÁ£ÌØÕ÷Ö¸ÎÆÍ¼Æ×¡£Ç°Êö»ÆÜοÅÁ£Ö¸ÎÆÍ¼Æ×µÄ²â¶¨·½·¨ÖУ¬¸ßЧҺÏàÉ«Æ×²â¶¨Ê±µÄÉ«Æ×Ìõ¼þΪ:É«Æ×ÖùΪAgilent ZORBAX SB-C18É«Æ×Öù£»Á÷¶¯ÏàAΪ¼×´¼£¬Á÷¶¯ÏàBΪ0.5%¼×ËáË®£¬A: B=2%-90%: 98%-10% ;Á÷ËÙ ImL.mirT1 ;ÖùΠ30 ¡ãC ;¼ì²â²¨³¤ 254nm¡£Ç°Êö»ÆÜοÅÁ£Ö¸ÎÆÍ¼Æ×µÄ²â¶¨·½·¨ÖУ¬¹©ÊÔÆ·ÈÜÒºµÄÖÆ±¸¹ý³ÌΪ:È¡»ÆÜοÅÁ£·ÛË飬¹ý60Ŀɸ£¬¾«ÃܳÆÈ¡·ÛÄ©1.0g,¼ÓÈë10%¼×´¼ÈÜÒºIOmLÈܽ⣬³¬ÉùÌáÈ¡5min£¬È»ºóÀäÈ´£¬²¹×ãËðÊ§ÖØÁ¿£¬Ò¡ÔÈ£¬Â˹ý£¬È¡ÐøÂËÒº¹ý0.45 ¦Ì m΢¿×ÂËĤ£¬¼´µÃ¹©ÊÔÆ·ÈÜÒº¡£Ç°Êö»ÆÜοÅÁ£Ö¸ÎÆÍ¼Æ×µÄ²â¶¨·½·¨ÖУ¬¶ÔÕÕÆ·ÈÜÒºµÄÖÆ±¸Îª:È¡¼õѹ¸ÉÔïÖÁºãÖØµÄëÈïÒì»ÆÍªÜÕ¡¢Ã¢±ú»¨ÜÕ¡¢Ã«ÈïÒì»ÆÍª¡¢Ã¢±ú»¨ËضÔÕÕÆ·£¬·Ö±ð¼ÓÈë¼×´¼£¬ÖƳÉÿImL·Ö±ðº¬0.562mgëÈïÒì»ÆÍªÜÕ¡¢0.254mgâ±ú»¨ÜÕ¡¢0.357mgëÈïÒì»ÆÍª¡¢0.274mgâ±ú»¨ËصĶÔÕÕÆ·ÈÜÒº¡£Ç°Êö»ÆÜοÅÁ£Ö¸ÎÆÍ¼Æ×µÄ²â¶¨·½·¨ÖУ¬²½Öè(3)Öзֱð¾«ÃÜÎüÈ¡¹©ÊÔÆ·ÈÜÒººÍ¶ÔÕÕÆ·ÈÜÒº¸÷5-20 ¦Ì L£¬ÓÅѡΪ10 ¦Ì L0ǰÊö»ÆÜοÅÁ£Ö¸ÎÆÍ¼Æ×µÄ²â¶¨·½·¨ÖУ¬Á÷¶¯ÏàÌݶÈÏ´Íѹý³ÌÖУ¬Ï´ÍÑÒºµÄA¡¢BÏà±ä»¯Îª:0 lOmin£¬AÏà 2%-2% £»10 15min£¬AÏà 2%-3% £»15 20min£¬AÏà 3%-10% ;20_25min£¬A Ïà 10%-15% £»25-40min, A Ïà 15%-20% £»40-45min, A Ïà 20%-30% £»45-50min, A Ïà 30%-40% £»50-60min, A Ïà 40%-56% £»60-72min, A Ïà 56%-70% £»72-80min, A Ïà 70%-90% £»80-90min, A Ïà90%-90%¡£Ç°Êö»ÆÜοÅÁ£Ö¸ÎÆÍ¼Æ×µÄ²â¶¨·½·¨ÖУ¬ÅäÖÆºÃµÄ¶ÔÕÕÆ·ÈÜÒº´¢´æÔÚ4VµÄ±ùÏäÖб¸Óá£ÈçǰËùÊö»ÆÜοÅÁ£Ö¸ÎÆÍ¼Æ×µÄ²â¶¨·½·¨µÃµ½µÄ»ÆÜοÅÁ£ÌØÕ÷Ö¸ÎÆÍ¼Æ×£»ËùÊöͼÆ×ÖеĹ²ÓзåΪ23¸ö¡£ÓëÏÖÓм¼ÊõÏà±È£¬±¾·¢Ã÷¾ßÓÐÒÔÏÂÓŵã:1.±¾·¢Ã÷²ÉÓøßЧҺÏàÉ«Æ×·¨£¬½¨Á¢»ÆÜοÅÁ£µÄÌØÕ÷Ö¸ÎÆÍ¼Æ×·ÖÎö·½·¨£¬³ýÁËÓ¦Óó£ÓõÄÏàËÆ¶È·ÖÎöÍ⣬»¹²ÉÓÃSimca-Pll.5Èí¼þ½øÐÐÖ÷³É·Ö·ÖÎö(PCA)£¬Í¬Ê±½áºÏSPSS¾ÛÀà·ÖÎö¶Ô¶à¸öÅú´ÎÑùÆ·½øÐÐϵͳ·ÖÎö£¬Äܸü¼ÓÈ«Ãæ¡¢¿Í¹Û¡¢¿ÆÑ§ÆÀ¼Û»ÆÜοÅÁ£µÄÖÊÁ¿£¬Îª»ÆÜοÅÁ£µÄºÏÀíÓÃÒ©¼°½øÒ»²½Ò©Ð§Ñо¿ÌṩÒÀ¾ÝÓë²Î¿¼¡£2.±¾·¢Ã÷½¨Á¢ÁË»ÆÜοÅÁ£HPLCÌØÕ÷Ö¸ÎÆÍ¼Æ×¹²ÓÐģʽ£¬±ê¶¨ÁË 23¸ö¹²Óз壬ָÈÏÁËÆäÖÐ4¸ö¹²Óз壬±ÈÏÖÓм¼ÊõÖ¸ÈϵÄÉ«Æ×·å¶à£¬±íÃ÷Æä½¨Á¢µÄÖ¸ÎÆÍ¼Æ×¼¼Êõº¬Á¿¸ß¡£Í³¼Æ½á¹ûÏÔʾ£¬²»Í¬Éú²úʱ¼ä30Åú´Î»ÆÜοÅÁ£±»·ÖΪÁ½´óÀ࣬Á½ÀàÑùÆ·µÄÉ«Æ×·å¹²ÓÐģʽ¾ßÓÐÒ»¶¨²îÒ죬ÕâÓë»ÆÜοÅÁ£ÔÁÏ»ÆÜÎÒ©²ÄÓÐÁ½¸ö·¨¶¨À´Ô´ÏàÎǺϡ£Òò¶ø±¾·¢Ã÷·½·¨¼ò±ã¡¢¿ì½Ý¡¢×¼È·¡¢Îȶ¨¡¢¿É¿¿£¬¿ÉÓÃÓÚ»ÆÜοÅÁ£ÑùÆ·µÄÖÊÁ¿¿ØÖÆ¡£3.ÓÉÓÚÖ¸ÎÆÍ¼Æ×²»ÊÇΪÁ˲ⶨij¸ö³É·ÖµÄ¾«È·º¬Á¿£¬¶øÊÇÒª³ä·Ö·´Ó³»¯Ñ§³É·ÖµÄÐÅÏ¢¡£Òò´Ë£¬±¾·¢Ã÷Ñ¡ÔñÔÚ254nm²¨³¤´¦½øÐвⶨ£¬Æä³ö·å½Ï¶à£¬·´Ó³µÄÐÅÏ¢½ÏÍêÈ«£»¸÷¸ö·åÎüÊÕÖµÁ¼ºÃ£¬»ùÏ߯½ÎÈ£¬Ò²±ÜÃâÁ˽ü×ÏÍâÔÓÖÊ·å½Ï´óÎüÊÕÇé¿ö¡£4.±¾·¢Ã÷·½·¨ËùµÃ»ÆÜοÅÁ£Ö¸ÎÆÍ¼Æ×µÄ·å¶à£¬·åÐκã¬Ò×ÓÚ¼ø±ð£¬ÏàËÆÐԸߣ¬×¼È·¿É¿¿¡£
ÏÂÃæ½áºÏʵʩÀýºÍ¸½Í¼¶Ô±¾·¢Ã÷×ö½øÒ»²½µÄ˵Ã÷¡£
ͼ1ÊÇ30Åú´Î»ÆÜοÅÁ£Ö¸ÎÆÍ¼Æ×µþ¼Óͼ£»Í¼2ÊÇ»ÆÜοÅÁ£ÑùÆ·µÄ¹²ÓÐģʽͼÆ×£»Í¼3ÊDz»Í¬Åú´Î»ÆÜοÅÁ£ÑùÆ·Ö÷³É·Ö·ÖÎö½á¹ûͼ£»Í¼4ÊDz»Í¬Åú´Î»ÆÜοÅÁ£ÑùÆ·¾ÛÀà·ÖÎö½á¹ûͼ£»Í¼5ÊÇ»ÆÜοÅÁ£ÑùÆ·µÄ¹²ÓÐģʽ²îÒìÐԱȽÏͼ£»Í¼6ÊÇÑ¡Óò»Í¬ÌáÈ¡ÈܼÁµÄ»ÆÜοÅÁ£HPLCÖ¸ÎÆÍ¼Æ×£»Í¼7ÊÇѡȡ²»Í¬ÌáÈ¡ÈܼÁÓÃÁ¿µÄ»ÆÜοÅÁ£HPLCÖ¸ÎÆÍ¼Æ×£»Í¼8ÊÇѡȡ²»Í¬³¬ÉùÌáȡʱ¼äµÄ»ÆÜοÅÁ£HPLCÖ¸ÎÆÍ¼Æ×£»Í¼9ÊÇÒÔ¼×´¼-ˮΪÁ÷¶¯Ïà½øÐÐÌݶÈÏ´ÍѵĻÆÜοÅÁ£HPLCÖ¸ÎÆÍ¼Æ×£»Í¼10ÊÇÒÔÒÒëæ-ˮΪÁ÷¶¯Ïà½øÐÐÌݶÈÏ´ÍѵĻÆÜοÅÁ£HPLCÖ¸ÎÆÍ¼Æ×£»Í¼11ÊÇÒÔ¼×´¼-0.5%¼×ËáˮΪÁ÷¶¯Ïà½øÐÐÌݶÈÏ´ÍѵĻÆÜοÅÁ£HPLCÖ¸ÎÆÍ¼Æ×£»Í¼12ÊÇÒÔ¼×´¼-0.1%¼×ËáˮΪÁ÷¶¯Ïà½øÐÐÌݶÈÏ´ÍѵĻÆÜοÅÁ£HPLCÖ¸ÎÆÍ¼Æ×£»Í¼13ÊÇÒÔ¼×´¼-0.1%ÒÒËáˮΪÁ÷¶¯Ïà½øÐÐÌݶÈÏ´ÍѵĻÆÜοÅÁ£HPLCÖ¸ÎÆÍ¼Æ×£»Í¼14ÊÇÓÃÉ«Æ×ÖùDiamonsil C18 (4.6mm*150mm, 25 ¦Ì m)²âµÃµÄ»ÆÃç¿ÅÁ£HPLCÖ¸ÎÆÍ¼Æ×£»Í¼15ÊÇÓÃÉ«Æ×ÖùHypersil ODS (4.0*250mm£¬5 ¦Ì m)²âµÃµÄ»ÆÜοÅÁ£HPLCÖ¸ÎÆÍ¼
-1'Tfe1Ô»£»Í¼16 ÊÇÓÃÉ«Æ×ÖùAgilent ZORBAX SB-C18C4.6*250mm, 5 ¦Ì m)²âµÃµÄ»ÆÜοÅÁ£HPLCÖ¸ÎÆÍ¼Æ×£»Í¼17 ÊÇÓÃÉ«Æ×Öù Agilent ZORBAX SB-C18C4.6*150mm, 5 ¦Ì m)²âµÃµÄ»ÆÜοÅÁ£ HPLCÖ¸ÎÆÍ¼Æ×£»Í¼18ÊÇÓÃÉ«Æ×ÖùPhecda C18 (4.6*250mm, 5 ¦Ì m)²âµÃµÄ»ÆÜοÅÁ£HPLCÖ¸ÎÆÍ¼Æ×£»Í¼ 19 ÊÇÔÚ 210nm¡¢230nm¡¢254nm¡¢270nm¡¢290nm¡¢300nm¡¢330nm ²¨³¤´¦½øÐвⶨµÄ»ÆÜοÅÁ£HPLCÖ¸ÎÆÍ¼Æ×£»Í¼20ÊÇÖùÎÂΪ25¡ãC¡¢30¡ãC¡¢35¡ãCʱ²âµÃµÄ»ÆÜοÅÁ£HPLCÖ¸ÎÆÍ¼Æ×¡£
¾ßÌåʵʩÀý·½Ê½±¾·¢Ã÷µÄʵʩÀý1:»ÆÜοÅÁ£Ö¸ÎÆÍ¼Æ×µÄ²â¶¨·½·¨°üÀ¨ÒÔϲ½Öè:(I)¹©ÊÔÆ·ÈÜÒºµÄÖÆ±¸:È¡»ÆÜοÅÁ£·ÛË飬¹ý60Ŀɸ£¬¾«ÃܳÆÈ¡·ÛÄ©l.0g£¬¼ÓÈë10%¼×´¼ÈÜÒºIOmLÈܽ⣬³¬ÉùÌáÈ¡5min£¬È»ºóÀäÈ´£¬²¹×ãËðÊ§ÖØÁ¿£¬Ò¡ÔÈ£¬Â˹ý£¬È¡ÐøÂËÒº¹ý0.45 ¦Ì m΢¿×ÂËĤ£¬¼´µÃ¹©ÊÔÆ·ÈÜÒº£»(2)¶ÔÕÕÆ·ÈÜÒºµÄÖÆ±¸:È¡¼õѹ¸ÉÔïÖÁºãÖØµÄëÈïÒì»ÆÍªÜÕ¡¢Ã¢±ú»¨ÜÕ¡¢Ã«ÈïÒì»ÆÍª¡¢Ã¢±ú»¨ËضÔÕÕÆ·£¬·Ö±ð¼ÓÈë¼×´¼£¬ÖƳÉÿImL·Ö±ðº¬0.562mgëÈïÒì»ÆÍªÜÕ¡¢0.254mgâ±ú»¨ÜÕ¡¢0.357mgëÈïÒì»ÆÍª¡¢0.274mgâ±ú»¨ËصĶÔÕÕÆ·ÈÜÒº(´¢´æÔÚ4¡ãCµÄ±ùÏäÖб¸ÓÃ)£»(3)²â¶¨:É«Æ×Ìõ¼þ:É«Æ×ÖùΪAgilent ZORBAX SB-C18É«Æ×Öù(5 ¦Ì mX4.6X 250mm);Á÷¶¯Ïà A Ϊ¼×´¼£¬Á÷¶¯Ïà B Ϊ 0.5% ¼×ËáË®£¬A: B=2%-90%: 98%-10% £»Á÷ËÙImL.miiT1 ;ÖùÎÂ30¡ãC ;¼ì²â²¨³¤254nm ;·Ö±ð¾«ÃÜÎüÈ¡¹©ÊÔÆ·ÈÜÒººÍ¶ÔÕÕÆ·ÈÜÒº¸÷10 ¦Ì L£¬×¢Èë¸ßЧҺÏàÉ«Æ×ÒDzⶨ£¬¼Ç¼ɫÆ×ͼ£»¹©ÊÔÆ·ÈÜҺעÈë¸ßЧҺÏàÉ«Æ×ÒǺó£¬ÒÔ¼×´¼-0.5%¼×ËáˮΪÁ÷¶¯Ïà½øÐÐÌݶÈÏ´ÍÑ£»ÌݶÈÏ´Íѹý³ÌÖУ¬Ï´ÍÑÒºµÄ¦¡¡¢BÏà±ä»¯Îª:
O lOmin£¬A Ïà 2%_2% ;10 15min£¬A Ïà 2%_3% ;15 20min£¬A Ïà 3%_10% £»20-25min, AÏà 10%-15% £»25-40min, A Ïà 15%-20% £»40-45min, A Ïà 20%-30% £»45-50min, A Ïà 30%-40% £»50-60min, A Ïà 40%-56% £»60-72min, A Ïà 56%-70% £»72-80min, A Ïà 70%-90% £»80-90min, A Ïà90%-90% £»(4)ÓÃÖ¸ÎÆÍ¼Æ×Èí¼þ¶ÔËùµÃµÄͼÆ×½øÐд¦Àí£¬¼´µÃµ½»ÆÜοÅÁ£ÌØÕ÷Ö¸ÎÆÍ¼Æ×¡£±¾·¢Ã÷µÄʵʩÀý2:»ÆÜοÅÁ£Ö¸ÎÆÍ¼Æ×µÄ²â¶¨·½·¨°üÀ¨ÒÔϲ½Öè:(I)¹©ÊÔÆ·ÈÜÒºµÄÖÆ±¸:È¡»ÆÜοÅÁ£·ÛË飬¹ý60Ŀɸ£¬¾«ÃܳÆÈ¡·ÛÄ©l.0g£¬¼ÓÈë10%¼×´¼ÈÜÒºIOmLÈܽ⣬³¬ÉùÌáÈ¡lOmin£¬È»ºóÀäÈ´£¬²¹×ãËðÊ§ÖØÁ¿£¬Ò¡ÔÈ£¬Â˹ý£¬È¡ÐøÂËÒº¹ý
0.45 ¦Ì m΢¿×ÂËĤ£¬¼´µÃ¹©ÊÔÆ·ÈÜÒº£»(2)¶ÔÕÕÆ·ÈÜÒºµÄÖÆ±¸:È¡¼õѹ¸ÉÔïÖÁºãÖØµÄëÈïÒì»ÆÍªÜÕ¡¢Ã¢±ú»¨ÜÕ¡¢Ã«ÈïÒì»ÆÍª¡¢Ã¢±ú»¨ËضÔÕÕÆ·£¬·Ö±ð¼ÓÈë¼×´¼£¬ÖƳÉÿImL·Ö±ðº¬0.562mgëÈïÒì»ÆÍªÜÕ¡¢0.254mgâ±ú»¨ÜÕ¡¢0.357mgëÈïÒì»ÆÍª¡¢0.274mgâ±ú»¨ËصĶÔÕÕÆ·ÈÜÒº(´¢´æÔÚ4¡ãCµÄ±ùÏäÖб¸ÓÃ)£»(3)²â¶¨:É«Æ×Ìõ¼þ:É«Æ×ÖùΪAgilent ZORBAX SB-C18É«Æ×Öù(5 ¦Ì mX4.6X 250mm);Á÷¶¯Ïà A Ϊ¼×´¼£¬Á÷¶¯Ïà B Ϊ 0.5% ¼×ËáË®£¬A: B=2%-90%: 98%-10% £»Á÷ËÙImL.miiT1 ;ÖùÎÂ30¡ãC ;¼ì²â²¨³¤254nm ;·Ö±ð¾«ÃÜÎüÈ¡¹©ÊÔÆ·ÈÜÒººÍ¶ÔÕÕÆ·ÈÜÒº¸÷
5¦Ì L£¬×¢Èë¸ßЧҺÏàÉ«Æ×ÒDzⶨ£¬¼Ç¼ɫÆ×ͼ£»¹©ÊÔÆ·ÈÜҺעÈë¸ßЧҺÏàÉ«Æ×ÒǺó£¬ÒÔ¼×´¼-0.5%¼×ËáˮΪÁ÷¶¯Ïà½øÐÐÌݶÈÏ´ÍÑ£»(4)ÓÃÖ¸ÎÆÍ¼Æ×Èí¼þ¶ÔËùµÃµÄͼÆ×½øÐд¦Àí£¬¼´µÃµ½»ÆÜοÅÁ£ÌØÕ÷Ö¸ÎÆÍ¼Æ×¡£±¾·¢Ã÷µÄʵʩÀý3:»ÆÜοÅÁ£Ö¸ÎÆÍ¼Æ×µÄ²â¶¨·½·¨°üÀ¨ÒÔϲ½Öè:(I)¹©ÊÔÆ·ÈÜÒºµÄÖÆ±¸:È¡»ÆÜοÅÁ£·ÛË飬¹ý60Ŀɸ£¬¾«ÃܳÆÈ¡·ÛÄ©1.0g£¬¼ÓÈë10%¼×´¼ÈÜÒºIOmLÈܽ⣬³¬ÉùÌáÈ¡2´Î£¬Ã¿´Î30min£¬È»ºóÀäÈ´£¬²¹×ãËðÊ§ÖØÁ¿£¬Ò¡ÔÈ£¬Â˹ý£¬È¡ÐøÂËÒº¹ý0.45 ¦Ì m΢¿×ÂËĤ£¬¼´µÃ¹©ÊÔÆ·ÈÜÒº£»(2)¶ÔÕÕÆ·ÈÜÒºµÄÖÆ±¸:È¡¼õѹ¸ÉÔïÖÁºãÖØµÄëÈïÒì»ÆÍªÜÕ¡¢Ã¢±ú»¨ÜÕ¡¢Ã«ÈïÒì»ÆÍª¡¢Ã¢±ú»¨ËضÔÕÕÆ·£¬·Ö±ð¼ÓÈë¼×´¼£¬ÖƳÉÿImL·Ö±ðº¬0.562mgëÈïÒì»ÆÍªÜÕ¡¢0.254mgâ±ú»¨ÜÕ¡¢0.357mgëÈïÒì»ÆÍª¡¢0.274mgâ±ú»¨ËصĶÔÕÕÆ·ÈÜÒº£»(3)²â¶¨:É«Æ×Ìõ¼þ:É«Æ×ÖùΪAgilent ZORBAX SB-C18É«Æ×Öù£»Á÷¶¯ÏàAΪ¼×´¼£¬Á÷¶¯ÏàBΪ0.5%¼×ËáË®£¬A: B=2%-90%: 98%-10% ;Á÷ËÙImL.mirT1 ;ÖùÎÂ30¡ãC ;¼ì²â²¨³¤254nm ;·Ö±ð¾«ÃÜÎüÈ¡¹©ÊÔÆ·ÈÜÒººÍ¶ÔÕÕÆ·ÈÜÒº¸÷20 ¦Ì L£¬×¢Èë¸ßЧҺÏàÉ«Æ×ÒDzⶨ£¬¼Ç¼ɫÆ×ͼ£»¹©ÊÔÆ·ÈÜҺעÈë¸ßЧҺÏàÉ«Æ×ÒǺó£¬ÒÔ¼×´¼-0.5%¼×ËáˮΪÁ÷¶¯Ïà½øÐÐÌݶÈÏ´ÍÑ£»(4)ÓÃÖ¸ÎÆÍ¼Æ×Èí¼þ¶ÔËùµÃµÄͼÆ×½øÐд¦Àí£¬¼´µÃµ½»ÆÜοÅÁ£ÌØÕ÷Ö¸ÎÆÍ¼Æ×¡£ÊµÑéÀý:1.ÒÇÆ÷ÓëÊÔÒ©1.1 ÒÇÆ÷°²½ÝÂ×1260¸ßЧҺÏàÉ«Æ×ÒÇ(Agilent G1315D-1260DAD¼ì²âÆ÷);KQ_250BÐͳ¬Éù²¨Çå½àÆ÷(À¥É½Êг¬ÉùÒÇÆ÷ÓÐÏÞ¹«Ë¾£¬¹¦ÂÊ250w);µç×Ó·ÖÎöÌìÆ½(A1205ÐÍ£¬Ã·ÌØÀÕ-ÍÐÀû¶àÒÇÆ÷£¬ÉϺ£ÓÐÏÞ¹«Ë¾)¡£
1.2 ÊÔÒ©¼×´¼£¬É«Æ×´¿£»ÒÒëæ£¬É«Æ×´¿£»Ë®ÎªÕôÁóË®£»ÆäËûÊÔ¼ÁΪ·ÖÎö´¿¡£Ã«ÈïÒì»ÆÍªÜÕ¡¢Ã«ÈïÒì»ÆÍª¡¢Ã¢±ú»¨ÜÕ¡¢Ã¢±ú»¨ËØ£¬ÆäÅúºÅ·Ö±ðΪ:M-020-110119£¬M-021-101215, C-018-101228, M-013-101116£¬³É¶¼Èð·Ò˼ÉúÎï¿Æ¼¼ÓÐÏÞ¹«Ë¾Ìṩ£»»ÆÜοÅÁ££¬¹óÖݺº·½ÖÆÒ©ÓÐÏÞ¹«Ë¾ÌṩµÄ2010-2011ÄêÉú²úµÄ30Åú´Î»ÆÜοÅÁ££¬ÆäÑùÆ·ÐÅÏ¢¼û±í
1±íI²»Í¬Åú´ÎÑùÆ·ÐÅÏ¢±í
ȨÀûÒªÇó
1.Ò»ÖÖ»ÆÜοÅÁ£Ö¸ÎÆÍ¼Æ×µÄ²â¶¨·½·¨£¬ÆäÌØÕ÷ÔÚÓÚ:°üÀ¨ÒÔϲ½Öè: (1)¹©ÊÔÆ·ÈÜÒºµÄÖÆ±¸:È¡»ÆÜοÅÁ£·ÛË飬¹ýɸ£¬³ÆÈ¡·ÛÄ©£¬¼Ó¼×´¼ÈÜÒºÈܽ⣬³¬ÉùÌáÈ¡I 2´Î£¬Ã¿´Î5 30min£¬È»ºóÀäÈ´£¬²¹×ãËðÊ§ÖØÁ¿£¬Ò¡ÔÈ£¬Â˹ý£¬È¡ÐøÂËÒº£¬¼´µÃ¹©ÊÔÆ·ÈÜÒº£» (2)¶ÔÕÕÆ·ÈÜÒºµÄÖÆ±¸:È¡¼õѹ¸ÉÔïÖÁºãÖØµÄëÈïÒì»ÆÍªÜÕ¡¢Ã¢±ú»¨ÜÕ¡¢Ã«ÈïÒì»ÆÍª¡¢Ã¢±ú»¨ËضÔÕÕÆ·£¬·Ö±ð¼ÓÈë¼×´¼ÖƳɶÔÕÕÆ·ÈÜÒº£» (3)²â¶¨:·Ö±ð¾«ÃÜÎüÈ¡¹©ÊÔÆ·ÈÜÒººÍ¶ÔÕÕÆ·ÈÜÒº£¬×¢Èë¸ßЧҺÏàÉ«Æ×ÒDzⶨ£¬¼Ç¼ɫÆ×ͼ£»¹©ÊÔÆ·ÈÜҺעÈë¸ßЧҺÏàÉ«Æ×ÒǺó£¬ÒÔ¼×´¼-0.5%¼×ËáˮΪÁ÷¶¯Ïà½øÐÐÌݶÈÏ´ÍÑ£» (4)ÓÃÖ¸ÎÆÍ¼Æ×Èí¼þ¶ÔËùµÃµÄͼÆ×½øÐд¦Àí£¬¼´µÃµ½»ÆÜοÅÁ£ÌØÕ÷Ö¸ÎÆÍ¼Æ×¡£
2.¸ù¾ÝȨÀûÒªÇó1ËùÊö»ÆÜοÅÁ£Ö¸ÎÆÍ¼Æ×µÄ²â¶¨·½·¨£¬ÆäÌØÕ÷ÔÚÓÚ:¸ßЧҺÏàÉ«Æ×²â¶¨Ê±µÄÉ«Æ×Ìõ¼þΪ:É«Æ×ÖùΪAgilent ZORBAX SB-C18É«Æ×Öù£»Á÷¶¯ÏàAΪ¼×´¼£¬Á÷¶¯ÏàBΪ0.5% ¼×ËáË®£¬A: B=2%-90%: 98%-10% ;Á÷ËÙ ImL.mirT1 ;ÖùΠ30¡ãC ;¼ì²â²¨³¤ 254nm¡£
3.¸ù¾ÝȨÀûÒªÇó1ËùÊö»ÆÜοÅÁ£Ö¸ÎÆÍ¼Æ×µÄ²â¶¨·½·¨£¬ÆäÌØÕ÷ÔÚÓÚ:¹©ÊÔÆ·ÈÜÒºµÄÖÆ±¸¹ý³ÌΪ:È¡»ÆÜοÅÁ£·ÛË飬¹ý60Ŀɸ£¬¾«ÃܳÆÈ¡·ÛÄ©1.0g£¬¼ÓÈë10%¼×´¼ÈÜÒºIOmLÈܽ⣬³¬ÉùÌáÈ¡5min£¬È»ºóÀäÈ´£¬²¹×ãËðÊ§ÖØÁ¿£¬Ò¡ÔÈ£¬Â˹ý£¬È¡ÐøÂËÒº¹ý0.45 ¦Ì m΢¿×ÂËĤ£¬¼´µÃ¹©ÊÔÆ·ÈÜÒº¡£
4.¸ù¾ÝȨÀûÒªÇó1ËùÊö»ÆÜοÅÁ£Ö¸ÎÆÍ¼Æ×µÄ²â¶¨·½·¨£¬ÆäÌØÕ÷ÔÚÓÚ:¶ÔÕÕÆ·ÈÜÒºµÄÖÆ±¸Îª:È¡¼õѹ¸ÉÔïÖÁºãÖØµÄëÈïÒì»ÆÍªÜÕ¡¢Ã¢±ú»¨ÜÕ¡¢Ã«ÈïÒì»ÆÍª¡¢Ã¢±ú»¨ËضÔÕÕÆ·£¬·Ö±ð¼ÓÈë¼×´¼£¬ÖƳÉÿImL·Ö±ðº¬0.562mgëÈïÒì»ÆÍªÜÕ¡¢0.254mgâ±ú»¨ÜÕ¡¢0.357mgëÈïÒì»ÆÍª¡¢0.274mgâ±ú»¨ËصĶÔÕÕÆ·ÈÜÒº¡£
5.¸ù¾ÝȨÀûÒªÇó1ËùÊö»ÆÜοÅÁ£Ö¸ÎÆÍ¼Æ×µÄ²â¶¨·½·¨£¬ÆäÌØÕ÷ÔÚÓÚ:²½Öè(3)Öзֱð¾«ÃÜÎüÈ¡¹©ÊÔÆ·ÈÜÒººÍ¶ÔÕÕÆ·ÈÜÒº¸÷5-20 ¦Ì L0
6.¸ù¾ÝȨÀûÒªÇó5ËùÊö»ÆÜοÅÁ£Ö¸ÎÆÍ¼Æ×µÄ²â¶¨·½·¨£¬ÆäÌØÕ÷ÔÚÓÚ:²½Öè(3)Öзֱð¾«ÃÜÎüÈ¡¹©ÊÔÆ·ÈÜÒººÍ¶ÔÕÕÆ·ÈÜÒº¸÷10 ¦Ì L0
7.¸ù¾ÝȨÀûÒªÇó2ËùÊö»ÆÜοÅÁ£Ö¸ÎÆÍ¼Æ×µÄ²â¶¨·½·¨£¬ÆäÌØÕ÷ÔÚÓÚ:Á÷¶¯ÏàÌݶÈÏ´Íѹý³ÌÖУ¬Ï´ÍÑÒºµÄ¦¡¡¢BÏà±ä»¯Îª:0 lOmin£¬AÏà2%_2% ;10 15min£¬AÏà2%_3% £»15 20min£¬A Ïà 3%_10% £»20-25min, A Ïà 10%_15% £»25-40min, A Ïà 15%_20% £»40-45min, AÏà 20%-30% £»45-50min, A Ïà 30%-40% £»50-60min, A Ïà 40%-56% £»60-72min, A Ïà 56%-70% £»72-80min,A Ïà 70%-90% £»80-90min, A Ïà 90%_90%¡£
8.¸ù¾ÝȨÀûÒªÇó4ËùÊö»ÆÜοÅÁ£Ö¸ÎÆÍ¼Æ×µÄ²â¶¨·½·¨£¬ÆäÌØÕ÷ÔÚÓÚ:ÅäÖÆºÃµÄ¶ÔÕÕÆ·ÈÜÒº´¢´æÔÚ4¡ãCµÄ±ùÏäÖб¸Óá£
9.ÈçȨÀûÒªÇó1 8ÖÐÈÎÒ»ÏîËùÊö»ÆÜοÅÁ£Ö¸ÎÆÍ¼Æ×µÄ²â¶¨·½·¨µÃµ½µÄ»ÆÜοÅÁ£ÌØÕ÷Ö¸ÎÆÍ¼Æ×¡£
10.¸ù¾ÝȨÀûÒªÇó9ËùÊöµÄ»ÆÜοÅÁ£ÌØÕ÷Ö¸ÎÆÍ¼Æ×£¬ÆäÌØÕ÷ÔÚÓÚ:ËùÊöͼÆ×ÖеĹ²ÓзåΪ23¸ö¡£
È«ÎÄÕªÒª
±¾·¢Ã÷¹«¿ªÁËÒ»ÖÖ»ÆÜοÅÁ£Ö¸ÎÆÍ¼Æ×µÄ²â¶¨·½·¨£¬°üÀ¨ÒÔϲ½Ö蹩ÊÔÆ·ÈÜÒºµÄÖÆ±¸¡¢¶ÔÕÕÆ·ÈÜÒºµÄÖÆ±¸¡¢¸ßЧҺÏàÉ«Æ×ÒDzⶨ¼°¶ÔÊý¾ÝºÍͼÆ×µÄ´¦Àí£¬ÒÔ¼°Óɸ÷½·¨µÃµ½µÄ»ÆÜοÅÁ£ÌØÕ÷Ö¸ÎÆÍ¼Æ×¡£ÓëÏÖÓм¼ÊõÏà±È£¬±¾·¢Ã÷ËùµÃ»ÆÜοÅÁ£Ö¸ÎÆÍ¼Æ×µÄ·å¶à£¬·åÐκ㬷å·ÖÀëЧ¹ûºÃ£¬·åÃæ»ý½Ï´ó£¬Ò×ÓÚ¼ø±ð£¬ÏàËÆÐԸߣ¬×¼È·¿É¿¿£»±¾·¢Ã÷·½·¨¼ò±ã¡¢¿ì½Ý¡¢×¼È·¡¢Îȶ¨¡¢¿É¿¿£¬¿ÉÓÃÓÚ»ÆÜοÅÁ£ÑùÆ·µÄÖÊÁ¿¿ØÖÆ£¬Äܸü¼ÓÈ«Ãæ¡¢¿Í¹Û¡¢¿ÆÑ§µØÆÀ¼Û»ÆÜοÅÁ£µÄÖÊÁ¿£¬Îª»ÆÜοÅÁ£µÄºÏÀíÓÃÒ©¼°½øÒ»²½Ò©Ð§Ñо¿ÌṩÁËÒÀ¾ÝÓë²Î¿¼¡£
Îĵµ±àºÅG01N30/02GK103149300SQ201310068779
¹«¿ªÈÕ2013Äê6ÔÂ12ÈÕ ÉêÇëÈÕÆÚ2013Äê3ÔÂ5ÈÕ ÓÅÏÈȨÈÕ2013Äê3ÔÂ5ÈÕ
·¢Ã÷ÕßÖÜÐÀ, ³Â»ª¹ú, ÕÔ³¬, ¹¨Ð¡¼û, ÕÔÑî, µË½Ü ÉêÇëÈË:¹óÖÝʦ·¶´óѧ