רÀûÃû³Æ£º¼ì²â¦Â-ÄÚõ£°·À࿹ÉúËØºÍÈý¾ÛÇè°·µÄÊÔ¼ÁºÐµÄÖÆ×÷·½·¨
¼¼ÊõÁìÓò£º
±¾ÊµÓÃÐÂÐÍÉæ¼°Ò»ÖÖ¼ì²â¦Â -ÄÚõ£°·ÀàºÍÈý¾ÛÇè°·µÄÊÔ¼ÁºÐ¼°·½·¨¡£
±³¾°¼¼Êõ£º
ÄÚõ£°·ÀàÒ©ÎïÊÇҽѧºÍÊÞҽѧÉϵÄÒ»ÀàÖØÒªµÄ¿¹¾úÒ©ÎӦÓÃÊ®·Ö¹ã·º¡£µ«ÓÉÓÚ¹ýÃô·´Ó¦¼°Ï¸¾ú²úÉúµÄÄÍÒ©ÐÔµÈÔÒò£¬Ðí¶à¹ú¼Ò¶¼¶Ô¸ÃÀàÒ©ÎïÔÚ¶¯ÎïÉϵÄʹÓü°ÔÚ¶¯ÎïÔ´ÐÔʳƷÖеIJÐÁô½øÐÐÁËÑϸñ¿ØÖÆ¡£ÃÀ¹úʳƷºÍÒ©Æ·¼à¶½¹ÜÀí¾Ö(FDA)µÄµ÷²é±íÃ÷²»×¢ÒâÐÝÒ©ÆÚ¡¢ÓÃÒ©²»ºÏÀí»òÊÇʹÓÃÎ¥½ûÒ©ÎÊǵ¼Ö¶¯ÎïÐÔʳƷÖп¹ÉúËØ²ÐÁôµÄÖ÷ÒªÔÒò¡£³¤ÆÚÉãÈ뺬¿¹ÉúËØµÄʳƷ£¬¿ÉÔì³ÉÒ©Îï»ýÀÛ£¬µ±´ïµ½Ò»¶¨Å¨¶Èºó£¬¾Í»á¶ÔÈËÌåµÄ½¡¿µ´øÀ´Ò»ÏµÁиºÃæÓ°Ï죬Èç²úÉú¹ýÃô·´Ó¦¡¢ÆÆ»µÎ¸³¦µÀ¾úȺƽºâºÍÔöǿϸ¾úÄÍÒ©ÐԵȡ£¸üÎªÖØÒªµÄÊÇ£¬ ¿¹ÉúËØ±»¹ýÁ¿Ê³È뽡¿µÈ˳¦µÀ£¬»áÆÆ»µ½¡¿µÈ˵ÄÕý³£¾úȺ»·¾³£¬µ¼ÖÂÈËÌåÃâÒßÁ¦µÄ½µµÍ£¬´Ó¶øÎ£º¦Ïû·ÑÕßµÄÉíÌ彡¿µ£¬Í¬Ê±Ò²Ó°ÏìÅ£Ä̼°ÈéÖÆÆ·µÄ³ö¿ÚóÒס£ÎÒ¹úµÄÔÁÏÈéÖЦÂ_ÄÚõ£°·À࿹ÉúËØ²ÐÁôÑÏÖØ£¬¶ÔÆä¿ªÕ¹¼à¿Ø¼ì²âÊÇÎÒ¹úÈéÆ·Òµ·¢Õ¹Öм«Îª¹Ø×¢µÄÎÊÌ⡣ŷÃË¡¢ ÃÀ¹úµÈ¶à¸ö¹ú¼Ò ¶¼Öƶ¨ÁËÅ£ÄÌ¡¢¼¡ÈâºÍÉöÔàµÈ¶¯ÎïÔ´ÐÔʳƷÖиÃÀàÒ©ÎïµÄ×î¸ß²ÐÁôÏÞÁ¿ (MRL)£¬ÎÒ¹úũҵ²¿235ºÅÎļþ¹«¸æ¶ÔÆäMRLÒ²×öÁËÑϸñ¹æ¶¨£¬¸ÃÀàÒ©ÎïÔÚÅ£ÄÌÖеÄMRLΪ ¦£ ¦¯¦¯¦Ì g/L£¬ÔÚ¼¡Èâ¡¢¸ÎÔàºÍÉöÔàÖеÄMRLΪ5(¦£ 000¦Ì g/kg¡£Èý¾ÛÇè°·(melamine)£¬¼ò³ÆÈý°·£¬ÊÇÒ»ÖÖÈýàºÀຬµªÔÓ»·Óлú»¯ºÏÎʳƷ¹¤ÒµÖг£ÐèÒª²â¶¨Ê³Æ·Öеĵ°°×Öʺ¬Á¿¡£ÓÉÓÚÖ±½Ó²âÁ¿µ°°×Öʼ¼Êõ±È½Ï¸´ÔÓ£¬ËùÒÔ³£ÓÿÊ϶¨µª·¨¼ä½ÓÈ·¶¨Ê³Æ·Öе°°×Öʵĺ¬Á¿¡£ÓÉÓÚÈý¾ÛÇè°·Óëµ°°×ÖÊÏà±Èº¬Óиü¸ß±ÈÀýµÄµªÔ×Ó£¬ ËùÒÔÈý¾ÛÇè°··Ç·¨Ìí¼Ó¼Á³£±»Ò»Ð©²»·¨ÉÌ··ÓÃ×÷ʳƷÌí¼Ó¼Á£¬ÒÔÌáÉýʳƷ¼ì²âÖеĵ°°×Öʺ¬Á¿Ö¸±ê¡£2008Äê8ÔÂÒÔÀ´£¬ÖйúÒ»Ð©Ò½ÔºÂ½Ðø¼ì²éµ½²¿·ÖÓ¤¶ù»¼Óе¨½áʯ£¬¶øÕâЩ»¼Óе¨½áʯµÄÓ¤¶ù¾ùºÈÁ˺¬ÓÐÈý¾ÛÇè°·µÄÄÌ·Û£¬ÕâÊÇÓ°ÏìÈ«¹úµÄ¡°Èý¹ÄÌ·Û¡±Ê¼þ¡£ÔÚÖ®ºóµÄµ÷²éÖз¢ÏÖ£¬ÓÐЩ²»·¨ÉÌÈËΪ»ñÈ¡¸ü¸ßµÄÀûÈó£¬Ö±½ÓÔÚÔÁÏÄÌÖÐÌí¼ÓÈý¾ÛÇè°·¡£ËæºóÔÚÈ«¹úÄÌ·ÛºÍҺ̬Ä̵ļì²âÖУ¬·¢ÏÖÊг¡ÉϺܴ󲿷ֲúÆ·Öоùº¬ÓÐÈý¾ÛÇè°·£¬µ¼ÖÂÁËÈ鯷ÐÐÒµµÄ³ÏÐÅΣ»ú£¬Ò²¸øÈËÃǵÄÈÕ³£Éú»îÔì³ÉÁ˾޴óµÄ¸ºÃæÓ°Ïì¡£ÎÒ¹úÎÀÉú²¿µÈÎ岿ÃÅÁªºÏ·¢²¼¹«¸æ£¬¹æ¶¨ÁËÎÒ¹úÓ¤¶ùÅ䷽ʳƷÖÐÈý¾ÛÇè°·ÏÞÁ¿ÖµÎªlmg/kg£¬ÆäËûʳƷÖÐÈý¾ÛÇè°·µÄÏÞÁ¿ÖµÎª 2. 5mg/kg¡£ÈéÖÆÆ·ÖеĿ¹ÉúËØºÍÈý¾ÛÇè°·Ìí¼Ó¼ÁµÄ²ÐÁôĿǰÒѾ³ÉΪÏû·ÑÕßËùÆÈÇйØ×¢µÄ½¹µãÎÊÌ⣬ΪʹţÄÌÖп¹ÉúËØºÍÈý¾ÛÇè°·Ìí¼Ó¼Á²ÐÁôÎÊÌâµÃµ½ÓÐЧ¼à¿Ø£¬·ûºÏ×î´ó²ÐÁôÏÞÁ¿±ê×¼£¬ÄÌÅ£³¡¡¢ÈéÖÆÆ·³§¡¢Õþ¸®¼à¶½²¿ÃÅ¡¢³öÈë¾³¼ì²â¡¢´óÐÍʳƷÁ÷ͨÁìÓòµÈ¶¼ÔÚŬÁ¦Ñ°Çó׼ȷ¿ÉÐеļì²â·½·¨£¬Ðí¶à´óÐÍ»¯Ñ§ÊÔ¼ÁºÍÒÇÆ÷¹«Ë¾Ò²ÖÂÁ¦ÓÚ¿ª·¢¦Â_ÄÚõ£°·ÀàºÍÈý¾ÛÇè°·²ÐÁô¼ì²âµÄ·½·¨ºÍÒÇÆ÷¡£Ä¿Ç°£¬Å£Ä̼°ÉúÏÊÄÌÖÐÄÚõ£°·ÀàºÍÈý¾ÛÇè°·²ÐÁôµÄ¼ì²â·½·¨ÒÀ¾Ý²»Í¬µÄ¼ì²âÔÀí£¬´óÌå¿É·ÖΪÈý´óÀà΢ÉúÎïÊÜ×è¼ì²â¡¢ÒÇÆ÷¼ì²â·¨¡¢ÃâÒßѧ·ÖÎö·¨¡£Î¢ÉúÎïÊÜ×è¼ì²â·½·¨,Ö÷ÒªÓÐÒÖ¾úȦÊÔÑé¡¢»ë×ǶÈÊÔÑé¡¢±äÉ«Ðͼì²â½ð±êÊÔ¼ÁºÐ·½·¨,΢ÉúÎï¼ì²â·½·¨¼ì²â³É±¾¼ÛµÍ£¬µ«ÊÇÁéÃô¶ÈµÍ¡¢ºÄʱ³¤¡¢ÄÍÒ©¾úµÄ´æÔÚÈÝÒ×µ¼ÖÂÎó¼ì£»ÒÇÆ÷¼ì²â·½·¨£¬ÊÇĿǰ¼ì²â¦Â -ÄÚõ£°·À࿹ÉúËØºÍÈý¾ÛÇè°·µÄÖ÷Òª·½·¨£¬ÓиßЧҺÏàÉ«Æ×·¨¡¢ÒºÏàÉ«Æ×-´®ÁªÖÊÆ×·¨¡¢ÆøÏàÉ«Æ×·¨¡¢ÆøÏàÉ«Æ×-´®ÁªÖÊÆ×·¨µÈ£¬ÒÇÆ÷¼ì²â·½·¨¾«È·¶È¸ß£¬µ«Æä´æÔÚ¼ì²â³É±¾¸ß£¬¼ì²âÉ豸¸´ÔÓ£¬¶Ô¼ì²âÈËÔ±µÄÒªÇó½Ï¸ß£¬¼ì²âºÄʱ³¤µÈȱµã£¬Ä¿Ç°³£ÓõÄÃâÒßѧ·ÖÎö·¨£¬Ö÷ҪΪøÁªÃâÒß¼ì²â·¨ºÍ·ÅÉäÃâÒß·ÖÎö·¨£¬Á½ÖÖ·½·¨µÄǰ´¦Àí¹ý³Ì½ÏΪ¸´ÔÓ£¬¶ÔÓÚÏÖ³¡´ó¹æÄ£É¸²éÑùÆ·»¹´æÔÚ×ÅÒ»¶¨¾ÖÏÞÐÔ¡£Öйú·¢Ã÷רÀû200810162588. 5 (CN101429241A)Öй«¿ªÁË¡°ÇàÃ¹ËØÓëÔØÌåµ°°×żÁª²úÎïºÍ¦Â-ÄÚõ£°·ÀàÇàÃ¹ËØ¿¹ÌåÖÆ±¸µÄ·½·¨¼°ÓÃ;¡±£¬¸Ã·½·¨ÖеÄÇàÃ¹ËØµ¥¿Ë¡¿¹ÌåÖÆ±¸µÄøÁªÃâÒßÊÔ¼ÁºÐ½öÄܼì²âÇàÃ¹ËØG¡¢°±ÜÐÇàÃ¹ËØ¡¢ôÈÜÐÇàÃ¹ËØÈýÖÖ¦Â-ÄÚõ£°·À࿹ÉúËØ£¬¼ì²âÒ©ÎïÖÖÀà ÉÙ£¬ÖƱ¸µÄ½ºÌå½ðÊÔÖ½Ìõ¼ì²âÁéÃô¶ÈµÍ¡£Ä¿Ç°ÃâÒß·ÖÎöÁìÓòȱ·¦Äܹ»Í¬Ê±¼ì²âÄÚõ£°·À࿹ÉúËØºÍÈý¾ÛÇè°·µÄ¼¼Êõ¼°ÆäÓ¦Óá£Òò´Ë£¬ÔÚʵ¼ùÖÐÓбØÒª½¨Á¢Ò»ÖÖ¼ì²âÒ©ÎïÖÖÀà¶à¡¢Ãô¸Ð¶È¸ß¡¢²Ù×÷¼òµ¥¡¢³É±¾µÍ¡¢Êʺϴó¹æÄ£ÍƹãµÄ¼ì²â·½·¨¡£
ʵÓÃÐÂÐÍÄÚÈݱ¾ÊµÓÃÐÂÐ͵ÄÄ¿µÄÊÇÌṩһÖÖÄܹ»Í¬Ê±¼ì²â¦Â_ÄÚõ£°·À࿹ÉúËØºÍÈý¾ÛÇè°·µÄÊÔ¼ÁºÐ¡£±¾ÊµÓÃÐÂÐÍËùÌṩµÄÊÔ¼ÁºÐ°üÀ¨Î¢¿×Ìõ¡¢Î¢¿×ÊÔ¼Á¡¢Î¢¿×ÈûºÍÊÔÖ½£¬ÊÔÖ½°üÀ¨µ×°å¡¢ÑùÆ·ÎüÊյ桢·´Ó¦Ä¤¡¢ÎüË®µæ¡¢±£»¤Ä¤£¬ÆäÒÀ´ÎÁ¬½Ó£»ËùÊö΢¿×ÊÔ¼ÁµÄ¶³¸ÉÓÐÍ·æßÀàÒ©ÎïÌØÒìÐÔ¿¹Ìå-½ºÌå½ð±ê¼ÇÎïºÍÈý¾ÛÇè°·ÌØÒìÐÔ¿¹Ìå-½ºÌå½ð±ê¼ÇÎ·´Ó¦Ä¤ÉϾßÓаü±»ÓÐÍ·æßÀàÒ©Îï-ÔØÌåµ°°×żÁªÎïÒÔ¼°Èý¾ÛÇè°·°ë¿¹Ô-ÔØÌåµ°°×żÁªÎïµÄÁ½Ìõ¼ì²âÏߺͰü±»Ñò¿¹Ê󿹿¹ÌåµÄÒ»ÌõÖÊ¿ØÏß¡£Á½Ìõ¼ì²âÏߺÍÒ»ÌõÖÊ¿ØÏß»¥ÏàÆ½ÐУ¬Èý¾ÛÇè°·¼ì²âÏß¿¿½üÑùÆ·ÎüÊÕµæÒ»¶Ë£¬ÖÊ¿ØÏß¿¿½üÎüË®µæÒ»¶Î¡£¦Â_ÄÚõ£°·ÀàÒ©Îï¼ì²âÏßÔÚÈý¾ÛÇè°·¼ì²âÏߺÍÖÊ¿ØÏßÖ®¼ä¡£ÆäÖÐÈý¾ÛÇè°·¼ì²âÏßÓüì²âÏß(T)±íʾ£¬¦Â-ÄÚõ£°·ÀàÒ©Îï¼ì²âÏßÓüì²âÏß
(B)±íʾ£¬ÖÊ¿ØÏßÓÃÖÊ¿ØÏß(C)±íʾ¡£ÖÊ¿ØÏß(C)¾àÀ뷴ӦĤµÄÄ©¶ËÓëÎüË®µæÏàÁ¬µÄÒ»¶ËΪ5-8mm,¼ì²âÏß(B)¾àÀëÖÊ¿ØÏß(C) 4_6mm£¬¼ì²âÏß(T)¾àÀë¼ì²âÏß(B) 4_6mm¡£ÔÚÊÔÖ½ÌõÉϱê¼ÇÓÐMAX±ê¼ÇÏßµÄÑùÆ·ÎüÊÕµæÉϵļì²â¶ËÕ³ÌùÓб£»¤Ä¤¡£ÔÚ×°Óж³¸ÉÓÐÍ·æßÀàÒ©ÎïÌØÒìÐÔ¿¹Ìå-½ºÌå½ð±ê¼ÇÎïºÍÈý¾ÛÇè°·µ¥¿Ë¡¿¹Ìå-½ºÌå½ð±ê¼ÇÎïµÄ΢¿×ÌõÉϾßÓÐ΢¿×Èû¡£ÎªÁ˱ãÓÚÔËÊäºÍʹÓã¬ÊÔ¼ÁºÐ»¹Óй̶¨Óþ߼°Æä¹Ì¶¨ÓÚÆäÖÐÓÃÓÚÊ¢×°ÊÔÖ½ÌõºÍ¶³¸ÉÓÐÍ·æßÀàÒ©ÎïÌØÒìÐÔ¿¹Ìå-½ºÌå½ð±ê¼ÇÎïºÍÈý¾ÛÇè°·ÌØÒìÐÔ¿¹Ìå-½ºÌå½ð±ê¼ÇÎïµÄ΢¿×ÌõµÄÊÔ¼ÁͰ£¬ËùÊöÊÔ¼ÁͰÉϾßÓÐÃÜ·â¸Ç¡£±¾ÊµÓÃÐÂÐÍÊÔ¼ÁºÐÖÐÊÔÖ½Ìõµ×°å¿ÉΪPVCµ×°å»òÆäËûÓ²Öʲ»ÎüË®µÄ²ÄÁÏ£»ÑùÆ·ÎüÊÕµæ¿ÉΪÎüÂËÖ½»òÂËÓÍÖ½£»ÎüË®µæÎªÎüˮֽ£»·´Ó¦Ä¤¿ÉΪÏõËáÏËÎ¬ËØÄ¤»ò´×ËáÏËÎ¬ËØÄ¤£»±£»¤Ä¤ÎªPE²ÄÖʱ£»¤Ä¤£»ÊÔ¼ÁºÐºÐÌåΪӲֽºÐ£»ÊÔ¼ÁͰΪËÜÁÏÊÔ¼ÁͰ£»¹Ì¶¨ÓþßΪӲÖÊÖ§³Å²ÄÁÏ¡£±¾ÊµÓÃÐÂÐÍÊÔ¼ÁºÐ¾ßÓÐÈçÏÂÓÐÒæÐ§¹ûI.ÁéÃô¶È¸ß´«Í³µÄÊÔÖ½¿¨¾ù¾ßÓй̶¨ÊÔÖ½ÌõµÄ¿¨¿Ç¹Ì¶¨×°ÖÃ,¿¨¿Ç¹Ì¶¨×°Öýô½ôµØ½«ÊÔÖ½Ìõ¹Ì¶¨ÆäÖУ¬ÔÚ¶ÔÑùÆ·¼ì²âʱ£¬ÓÉÓÚ¿¨¿ÇÓëÊÔÖ½Ìõ½áºÏµÄ±È½Ï½ôÃÜ£¬ÖÂʹ¼ì²âÑùÆ·ÈÜÒº²»Äܹ»³ä·ÖÉøÍ¸µ½ÑùÆ·ÎüÊÕµæÒ»¶Î£¬²¢ÔÚÊÔÖ½ÌõÉϽøÐвãÎöµÄËÙ¶ÈÊ®·Ö»ºÂý£¬µ¼Ö¼ì²âÑùÆ·ÖеÄÒ©Îï²»Äܹ»Óë¼ì²âÏßÉϰü±»µÄ°ü±»Ô³ä·Ö½Ó´¥£¬ÖÂʹÊÔÖ½¿¨µÄÁéÃô¶ÈϽµ¡£±¾ÊµÓÃÐÂÐÍÊÔ¼ÁºÐ±È´«Í³ÊÔÖ½¿¨µÄ¼ì²âÁéÃô¶È¸ß£¬ÓÉÓÚ±¾ÊµÓÃÐÂÐÍÊÔ¼ÁºÐµÄÊÔÖ½ÌõΪÂãÌõ×´£¬ÎÞÐè¿ÇÌå½ô¹Ì×°Ö㬼ì²âÑùÆ·¿ÉÒÔÖ±½Ó½Ó´¥ÊÔÖ½ÌõµÄÑùÆ·ÎüÊÕµæÒ»¶Ë£¬²¢ÇÒÑùÆ·ÔÚÊÔÖ½ÌõÉϵIJãÎöËٶȱȽϿ죬ʹÑùÆ·ÖеĴý¼ìÒ©ÎïÄܹ»³ä·ÖµÄÓë¼ì²âÏßÉϰü±»µÄ°ü±»Ô½áºÏ£¬ ´Ó¶øÌá¸ßÁËÊÔÖ½ÌõµÄ¼ì²âÁéÃô¶È¡£¦Â-ÄÚõ£°·À࿹ÉúËØºÍÈý¾ÛÇè°·¼ì²âÊÔ¼ÁºÐÒÔ½ºÌå½ð±ê¼Ç¸ßÇ׺ÍÁ¦µÄµ¥¿Ë¡¿¹ÌåΪ»ù´¡ÖƱ¸¶ø³É£¬½ð±ê¿¹ÌåÖнð¿ÅÁ£Ó뿹Ìå·Ö×ÓÖ®¼äÎÞ¼Û½¡Ðγɣ¬¶þÕßͨ¹ýÒìÐÔµçºÉ¼äµÄ·¶µÂ»ªÁ¦Ïà½áºÏ£¬½ºÌå½ð¶Ôµ¥¿Ë¡¿¹ÌåÌØÒìÐÔºÍÇ׺ÍÁ¦Ó°ÏìºÜС£¬ÇÒ¾ßÓнϸߵıê¼ÇÂÊ¡£ÆäÖеÄÍ·æßÀàÒ©Îïµ¥¿Ë¡¿¹Ìå-½ºÌå½ð±ê¼ÇÎïºÍÈý¾ÛÇè°·µ¥¿Ë¡¿¹Ìå-½ºÌå½ð±ê¼ÇÎï¶³¸ÉÔÚ΢¿×ÊÔ¼ÁÌõÖУ¬ÔÚ¼ì²â¹ý³ÌÖУ¬Äܹ»Ê¹½ð±ê¿¹ÌåÓë´ý¼ìÑùÆ·Òº³ä·Ö½Ó´¥£¬³ä·Ö·´Ó¦£¬´Ó¶ø¼õÉÙÎó²î£¬Ôö¼ÓÕû¸öÌåϵµÄ·´Ó¦ÁéÃô¶È¡£Òò´Ë£¬±¾ÊµÓÃÐÂÐÍÊÔ¼ÁºÐ¾ßÓнϸߵÄÌØÒìÐÔºÍÁéÃô¶È¡£±¾ÊÔ¼ÁºÐ¶Ô¦Â-ÄÚõ£°·ÀàÒ©Îï¼ì²âÁéÃô¶È·Ö±ðΪÇàÃ¹ËØ G 2¦Ì g/L¡¢°±½ÚÇàÃ¹ËØ3 ¦Ì 8/1¡¢°¢ÄªÎ÷ÁÖ4 4 g/L¡¢±½ßòÇàÃ¹ËØ6¦Ì g/L¡¢ÁÚÇàÃ¹ËØ6¦Ì g/L¡¢Ë«ÇàÃ¹ËØ6 ¦Ì g/L¡¢ÝÁ·òÎ÷ÁÖ20 ¦Ì g/L¡¢Í·æßàÏá20 ¦Ì g/L¡¢Í·æßßßͪ40 ¦Ì g/L¡¢Í·æßÇúËÉ50 ¦Ì g/ L¡¢Í·æßàçß»90 ¦Ì g/L¡¢Í·æßÂåÄþ10 ¦Ì g/L ;¶ÔÈý¾ÛÇè°·¼ì²âÁéÃô¶ÈΪ50 ¦Ì g/L¡£2.²Ù×÷¼ò±ã¿ìËÙ¶ÔÓÚÒÔÍùµÄÊÔÖ½¿¨¼ì²âÔÁÏÄ̱ØÐëÒª½«ÔÁÏÄ̼ÓÈëÏ¡ÊÍÒº½øÐÐÏ¡ÊͲÅÄܹ»µãÑù¼ì²â£¬ÔÒòÊÇÓÉÓڹ̶¨ÊÔÖ½ÌõµÄËÜÁÏ¿ÇÌå½ô½ôµØ°ÑÊÔÖ½Ìõ¹Ì¶¨ÔÚ¿ÇÌåÄÚ£¬¶øÔÁÏÄ̱ȽÏÕ³³í£¬ËùÒÔÖ±½Ó½«ÔÁÏÄÌÔÚÊÔÖ½¿¨µÄ¼ÓÑù¿×½øÐеãÑù¼ì²âµÄ»°£¬ÓÉÓÚ¿ÇÌåÓëÊÔÖ½Ìõ½áºÏ½ôÃÜÒÔ¼°ÄÌÒºÕ³³íËùÖ£¬ÔÁÏÄÌÑùÆ·ÎÞ·¨ÍêÈ«ÉøÍ¸µ½ÑùÆ·ÎüÊÕµæºÍ½áºÏÎïÊͷŵæÖУ¬²¢ÇÒÔÚÊÔÖ½ÌõÉϵIJãÎöËٶȱȽϻºÂý£¬ÒÔÖÁÓÚ ÎÞ·¨¼ì²â£¬¹ÊÒª½«ÔÁÏÄ̼ÓÈëÏ¡ÊÍÒº½øÐÐÏ¡Êͺ󷽿ɼì²â¡£Ê¹Óñ¾ÊµÓÃÐÂÐÍÊÔ¼ÁºÐ¼ì²âʱÎÞÐèÈÎºÎÆäËüÊÔ¼Á£¬¼ì²âÔÁÏÄÌʱ£¬Ò²²»ÐèÒª¶ÔÔÁÏÄ̽øÐÐÏ¡ÊÍ£¬ÔÒòÓÉÓÚ±¾ÊµÓÃÐÂÐÍÊÔ¼ÁºÐÖеÄÊÔÖ½ÌõΪÂãÌõ×´£¬¼´ÎÞÐèÓÿÇÌå¶ÔÆä½øÐй̶¨£¬Ö»Òª½«´ý¼ìÑùÆ·ÈÜÒºÖ±½ÓµÎ¼ÓÓÚ¶³¸ÉÓÐÍ·æßÀàÒ©Îïµ¥¿Ë¡¿¹Ìå-½ºÌå½ð±ê¼ÇÎïºÍÈý¾ÛÇè°·µ¥¿Ë¡¿¹Ìå-½ºÌå½ð±ê¼ÇÎïµÄ΢¿×ÖУ¬»ìÔȺ󣬷õÓý5min£¬ÔÙ½«ÊÔÖ½±êÓÐMAXÏß±ê¼Ç¶ËÏòÏ£¬²åÈë·õÓýºóµÄ΢¿×ÊÔ¼ÁÖУ¬ÊÔÖ½Ìõ¿ÉÒÔÓëÑùÆ·ÈÜÒº³ä·Ö½Ó´¥£¬5minºó¹Û²ì½á¹û¡£¶ÔÓÚÅ£ÄÌÑùÆ·µÄ¼ì²â£¬±¾ÊµÓÃÐÂÐÍÊÔ¼ÁºÐ±È´«Í³ÊÔÖ½¿¨¼ì²âʱ¼ä¶Ì£¬ÓÉÓÚ±¾ÊµÓÃÐÂÐÍÊÔ¼ÁºÐÎÞÐè¶ÔÑùÆ·½øÐÐÏ¡ÊÍ£¬¿ÉÖ±½Ó¶ÔÑùÆ·½øÐмì²â¡£3.ÏÔʾ¼ì²â½á¹ûÐÎÏó¡¢Ö±¹Û׼ȷ¡£¼ì²â½á¹ûµÄÅж¨ÊÇÒÔÊÔÖ½ÌõµÄÁ½Ìõ¼ì²âÏߺÍÒ»ÌõÖÊ¿ØÏßÊÇ·ñÏÔÉ«×÷ΪÒõÐÔºÍÑôÐÔÅжϱê×¼¡£Èç¹û·´Ó¦Ä¤ÉϵÄÈý¾ÛÇè°·¼ì²âÏßÏÔʾһÌõºìÉ«Ìõ´ø£¬ÄÚõ£°·ÀàÒ©Îï¼ì²âÏßÒ²ÏÔʾһÌõºìÉ«Ìõ´ø£¬Í¬Ê±ÖÊ¿ØÏßÒ²ÏÔʾһÌõºìÉ«Ìõ´ø£¬ ±íʾÑùÆ·ÖЦÂ-ÄÚõ£°·ÀàÒ©ÎïºÍÈý¾ÛÇè°·¾ù·Ö±ðµÍÓÚÊÔ¼ÁºÐ¶Ô¦Â-ÄÚõ£°·ÀàÒ©ÎïºÍÈý¾ÛÇè°·µÄ×îµÍ¼ì²âÏÞ£»Èç¹û·´Ó¦Ä¤ÉϵÄÈý¾ÛÇè°·¼ì²âÏßÏÔʾһÌõºìÉ«Ìõ´ø£¬Í¬Ê±ÖÊ¿ØÏßÒ²ÏÔʾһÌõºìÉ«Ìõ´ø£¬¶ø¦Â-ÄÚõ£°·ÀàÒ©Îï¼ì²âÏß²»ÏÔÉ«£¬±íʾ¼ì²âÑùÆ·ÖÐÖ»ÓЦÂ-ÄÚõ£°·ÀàÒ©ÎﺬÁ¿µÈÓÚ»ò¸ßÓÚÊÔ¼ÁºÐ¶Ô¦Â_ÄÚõ£°·ÀàÒ©ÎïµÄ×îµÍ¼ì²âÏÞ£»Èç¹ûÔÚ·´Ó¦Ä¤ÉϵĦÂ_ÄÚõ£°·ÀàÒ©Îï¼ì²âÏßÏÔʾһÌõºìÉ«Ìõ´ø£¬Í¬Ê±ÖÊ¿ØÏßÒ²ÏÔʾһÌõºìÉ«Ìõ´ø£¬¶øÈý¾ÛÇè°·¼ì²âÏß²»ÏÔÉ«£¬±íʾ¼ì²âÑùÆ·ÖÐÖ»ÓÐÈý¾ÛÇè°·º¬Á¿µÈÓÚ»ò¸ßÓÚÊÔ¼ÁºÐ¶ÔÈý¾ÛÇè°·µÄ×îµÍ¼ì²âÏÞ£»Èç¹û·´Ó¦Ä¤ÉÏÖ»ÓÐÖÊ¿ØÏßÏÔʾºìÉ«Ìõ´ø£¬¶øÈý¾ÛÇè°·¼ì²âÏߺͦÂ_ÄÚõ£°·ÀàÒ©Îï¼ì²âÏß¾ù²»ÏÔʾºìÉ«Ìõ´ø£¬±íʾ¼ì²âÑùÆ·ÖеÄÈý¾ÛÇè°·ºÍ¦Â_ÄÚõ£°·ÀàÒ©ÎïµÄº¬Á¿Í¬Ê±µÈÓÚ»ò¸ßÓÚÊÔ¼ÁºÐ¶ÔÈý¾ÛÇè°·ºÍ¦Â_ÄÚõ£°·ÀàÒ©ÎïµÄ×îµÍ¼ì²âÏÞ£»Èç¹û·´Ó¦Ä¤ÉϵÄÖÊ¿ØÏßûÓÐÏÔʾºìÉ«Ìõ´ø£¬ÎÞÂÛÈý¾ÛÇè°·¼ì²âÏߺÍ(»ò)¦Â -ÄÚõ£°·ÀàÒ©Îï¼ì²âÏßÊÇ·ñÏÔʾºìÉ«Ìõ´ø£¬ÊÔÖ½Ìõ¼ì²â½á¹û¾ùÊÓΪÎÞЧ¡£½á¹ûÅж¨ÐÎÏó¡¢Ö±¹Û¡¢×¼È·¡¢¼òµ¥Ã÷ÁË£¬²»ÈÝÒ׳öÏÖ½á¹ûÎóÅС£4.³É±¾µÍ£¬Í¶×ÊÉÙʹÓñ¾ÊµÓÃÐÂÐÍÊÔ¼ÁºÐ£¬²»ÐèÁíÅäÒÇÆ÷É豸¼°ÆäËûÊÔ¼Á£¬Ò²²»ÐèÒª¹Ì¶¨ÊÔÖ½ÌõµÄ¿¨¿Ç¹Ì¶¨×°Ö㬳ɱ¾µÍÁ®£¬Í¶×ÊÉÙ£¬¼ûЧ¿ì£¬Ê¹ÏÖ³¡¼ì²âÒ»²½µ½Î»¡£5.Ò×ÓÚ´ó·¶Î§ÍƹãÓ¦ÓÃÊÔ¼ÁºÐ²Ù×÷¼òµ¥£¬ÄܽϺÃÂú×㲻ͬ²ã´ÎÈËÔ±µÄÐèÒª£¬Èç
רҵ»¯Ñé¡¢ÎÀÉú·ÀÒß¡¢ÖÊÁ¿¼à²â¡¢ÈéÒµÆóÒµµÈ£¬¾ßÓйãÀ«µÄÊг¡Ç°¾°ºÍ½Ï´óµÄ¾¼Ã¡¢Éç»áЧ
.¡¢/¡ö
Mo
ͼIΪ±¾ÊµÓÃÐÂÐÍÊÔÖ½Ìõ½á¹¹ÆÊÃæÍ¼£»Í¼2Ϊ±¾ÊµÓÃÐÂÐÍÊÔÖ½Ìõ¸©ÊÓͼ£» ͼ3Ϊ±¾ÊµÓÃÐÂÐͶ³¸ÉÓнð±ê¿¹ÌåµÄ΢¿×Ìõ£»Í¼4Ϊ±¾ÊµÓÃÐÂÐÍ΢¿×Èû¸©ÊÓͼ£»Í¼5Ϊ±¾ÊµÓÃÐÂÐÍ΢¿×Èû²àÊÓͼ£»Í¼6a¡¢6b¡¢6c¡¢6d¡¢6el¡¢6e2¡¢6e3¡¢6e4Ϊ±¾ÊµÓÃÐÂÐÍÊÔÖ½Ìõ¼ì²â½á¹ûÅж¨Í¼£»Í¼7Ϊ±¾ÊµÓÃÐÂÐÍÊÔ¼ÁͰ½á¹¹Ê¾Òâͼ£»Í¼8Ϊ±¾ÊµÓÃÐÂÐ͹̶¨Óþߵĸ©ÊÓͼ£»Í¼9Ϊ±¾ÊµÓÃÐÂÐͺÐÌåÓë¹Ì¶¨ÓþߵIJàÊÓͼ¡£
¾ßÌåʵʩ·½Ê½
Ò»¡¢¦Â -ÄÚõ£°·À࿹ÉúËØºÍÈý¾ÛÇè°·¼ì²âÊÔ¼ÁºÐµÄ×é×°ÖÆ±¸I)ÊÔÖ½ÌõÑùÆ·ÎüÊÕµæI¡¢·´Ó¦Ä¤2¡¢ÎüË®µæ3ºÍ±£»¤Ä¤7ÒÀ´Î°´Ë³Ðòð¤ÌùÔÚËùÊöµ×°å6ÉÏ£¬ÑùÆ·ÎüÊÕµæµÄÄ©¶ËÓ뷴ӦĤʼ¶ËÏàÁ¬£¬·´Ó¦Ä¤µÄÄ©¶ËÓëÎüË®µæÏàÁ¬£¬ÑùÆ·ÎüÊÕµæµÄʼ¶ËÓëµ×°åµÄʼ¶Ë¶ÔÆë£¬ÎüË®µæµÄÄ©¶ËÓëµ×°åµÄÄ©¶Ë¶ÔÆë£¬±£»¤Ä¤7¸²¸ÇÔÚÑùÆ·ÎüÊÕµæµÄ¼ì²â¶Ë£¬ÔÚ¼ì²â¶Ë±£»¤Ä¤ÉÏÓ¡ÓÐMAX±ê¼ÇÏß¡£·´Ó¦Ä¤ÉϵÄÁ½Ìõ¼ì²âÏߺÍÒ»ÌõÖÊ¿ØÏß»¥ÏàÆ½ÐУ¬²¢ÇÒÓëÊÔÖ½ÌõµÄ³¤Ïà´¹Ö±¡£¼ì²âÏß4-1¼´Îª¼ì²âÏß(¦³)£¬¿¿½üÑùÆ·ÎüÊÕµæÒ»¶Ë£¬ÖÊ¿ØÏß5¼´ÎªÖÊ¿ØÏß(C)£¬¿¿½üÎüË®µæÒ»¶Ë£¬¼ì²âÏß4-2¼´Îª¼ì²âÏß(B)£¬ÔÚ¼ì²âÏß(T)ºÍÖÊ¿ØÏß(C)Ö®¼ä¡£ÖÊ¿ØÏß(C)¾àÀ뷴ӦĤµÄÄ©¶ËÓëÎüË®µæÏàÁ¬µÄÒ»¶ËΪ5-8mm£¬¼ì²âÏß(B)¾àÀëÖÊ¿ØÏß(C) 4_6mm£¬¼ì²âÏß(T)¾àÀë¼ì²âÏß(B) 4-6mm¡£¼ì²âÏß(T)°ü±»ÓÐÈý¾ÛÇè°·°ë¿¹Ô_ÔØÌåµ°°×żÁªÎ¼ì²âÏß(B)°ü±»ÓÐÍ·æßÀàÒ©Îï-ÔØÌåµ°°×żÁªÎÖÊ¿ØÏß(C)°ü±»ÓÐÑò¿¹Ê󿹿¹Ìå¡£µ×°åΪPVCµ×°å£¬ÑùÆ·ÎüÊÕµæÎªÎüÂËÖ½£¬·´Ó¦Ä¤ÎªÏõËáÏËÎ¬ËØÄ¤£¬ÎüË®µæÎªÎüˮֽ£¬±£»¤Ä¤ÎªPE²ÄÖʱ£»¤Ä¤¡£2)¶³¸ÉÓнð±ê¿¹ÌåµÄ΢¿×Ìõ ËùÊö΢¿×Ìõ8µÄµ×²¿¶³¸ÉÓÐÍ·æßÀàÒ©ÎïÌØÒìÐÔ¿¹Ìå-½ºÌå½ð±ê¼ÇÎïºÍÈý¾ÛÇè°·ÌØÒìÐÔ¿¹Ìå-½ºÌå½ð±ê¼ÇÎ¼´½ð±ê¿¹Ìå9¡£Î¢¿×Ìõ8¾ßÓÐ΢¿×Èû10¡£3)½«I)ÊÔÖ½ÌõºÍ2)΢¿×Ìõ×°Èë¾ßÓÐÃÜ·âÈûµÄÊÔ¼ÁͰ11ÖУ¬½«ÊÔ¼ÁͰ·ÅÖÃÓÚÊÔ¼ÁºÐºÐÌå13ÄڵĹ̶¨Óþß12ÖС£¶þ¡¢ÊÔ¼ÁºÐµÄʹÓÃÈ¡´ý¼ìÅ£ÄÌÑùÆ·ÈÜÒºµÎ¼Ó200 ¦Ì Iµ½Î¢¿×ÊÔ¼ÁÖУ¬»ìÔȺ󣬷õÓý5min£¬½«ÊÔÖ½±êÓÐMAXÏß±ê¼Ç¶ËÏòÏ£¬²åÈëõrÓýºóµÄ΢¿×ÊÔ¼Áºó¼ÆÊ±£¬5minºó¹Û²ì½á¹û¡£Èý¡¢¼ì²â½á¹û·ÖÎö[0036]¦Â -ÄÚõ£°·À࿹ÉúËØºÍ(»ò)Èý¾ÛÇè°·ÔÚ¼ì²âÑôÐÔÑùÆ·Öеĺ¬Á¿µÈÓÚ»ò¸ßÓÚÊÔ¼ÁºÐµÄ¶ÔÉÏÊöÁ½ÀàÒ©ÎïµÄ¼ì²âÏÞʱ£¬Í·æßÀàÒ©ÎïÌØÒìÐÔ¿¹Ìå-½ºÌå½ð±ê¼ÇÎïºÍ(»ò)Èý¾ÛÇè°·ÌØÒìÐÔ¿¹Ìå-½ºÌå½ð±ê¼ÇÎïÓë¶ÔÓ¦µÄÒ©Îï½áºÏ£¬½ð±ê¿¹ÌåÉϵĿ¹Ô½áºÏλµã±»·â±Õ£¬´Ó¶øÔÚ¼ì²âÏßÉÏÒòΪ¾ºÕù·´Ó¦£¬²»»áÓë¶ÔÓ¦Ò©Îï-ÔØÌåµ°°×żÁªÎï½áºÏ¶ø²»³öÏÖºìÉ«Ìõ´ø¡£ÒõÐÔÑùÆ·ÔÚ¼ì²â¹ý³ÌÖÐÓÉÓÚȱÉÙ¿¹Ô¿¹Ì徺Õù·´Ó¦£¬½«»áÔÚÁ½Ìõ¼ì²âÏߺÍÒ»ÌõÖÊ¿ØÏßÉϳöÏÖºìÉ«Ìõ´ø¡£Èçͼ6Ëùʾ£¬ÆäÖмì²âÏß(T)±íʾÈý¾ÛÇè°·¼ì²âÏߣ¬¼ì²âÏß(B)±íʾ¦Â-ÄÚõ£°·ÀàÒ©Îï¼ì²âÏߣ¬ÒõÐÔ½á¹ûÓñíʾ£¬ÑôÐÔ½á¹ûÓá° + ¡±±íʾ¡£¦Â -ÄÚõ£°·ÀàÒ©ÎïºÍÈý¾ÛÇè°·¾ù³ÊÒõÐÔµ±ÖÊ¿ØÏß(C)ÏÔʾһÌõºìÉ«Ìõ´ø£¬¼ì²âÏß (T)ºÍ¼ì²âÏÞ(B)Ҳͬʱ·Ö±ð³öÏÖºìÉ«Ìõ´ø£¬¦Â-ÄÚõ£°·ÀàÒ©ÎïºÍÈý¾ÛÇè°·¾ù³ÊÒõÐÔ¡£Èçͼ 6aËùʾ¡£¦Â -ÄÚõ£°·ÀàÒ©Îï»òÈý¾ÛÇè°·³ÊÑôÐÔµ±ÖÊ¿ØÏß(C)ÏÔʾһÌõºìÉ«Ìõ´ø£¬Í¬Ê±¼ì²âÏß(T)³öÏÖºìÉ«Ìõ´ø£¬¶ø¼ì²âÏß(B)²»³öÏÖºìÉ«Ìõ´øÊ±£¬¦Â-ÄÚõ£°·ÀàÒ©Îï³ÊÑôÐÔ£¬Èçͼ6b Ëùʾ£»µ±ÖÊ¿ØÏß(C)ÏÔʾһÌõºìÉ«Ìõ´ø£¬Í¬Ê±¼ì²âÏß(B)³öÏÖºìÉ«Ìõ´ø£¬¶ø¼ì²âÏß(T)²»³öÏÖºìÉ«Ìõ´øÊ±£¬Èý¾ÛÇè°·³ÊÑôÐÔ£¬Èçͼ6cËùʾ¡£¦Â -ÄÚõ£°·ÀàÒ©ÎïºÍÈý¾ÛÇè°·¾ù³ÊÑôÐÔµ±ÖÊ¿ØÏß(C)ÏÔʾһÌõºìÉ«Ìõ´ø£¬¶ø¼ì²âÏß(T)ºÍ¼ì²âÏß(B)¶¼²» ³öÏÖºìÉ«Ìõ´øÊ±£¬¦Â -ÄÚõ£°·ÀàÒ©ÎïºÍÈý¾ÛÇè°·¾ù³ÊÑôÐÔ£¬Èçͼ6d Ëùʾ¡£ÊÔÖ½ÌõÎÞЧµ±ÖÊ¿ØÏß(C)²»ÏÔʾ³öºìÉ«Ìõ´ø£¬ÔòÎÞÂÛ¼ì²âÏß(T)ºÍ(»ò)¼ì²âÏß (B)ÏÔʾ³öºìÉ«Ìõ´øÓë·ñ£¬¸ÃÊÔÖ½ÌõÅÐΪÎÞЧ£¬Èçͼ6el_6e4Ëùʾ¡£ËÄ¡¢¼ì²âÊÔ¼ÁºÐÖÐÓõ½µÄ²ÄÁÏÖÆ±¸·½·¨ÈçÏÂI¡¢¿¹ÔµÄÖÆ±¸I)¡¢Í·æßÀàÒ©Îï-ÔØÌåµ°°×żÁªÎïµÄºÏ³ÉÓë¼ø¶¨Í·æßÀàÒ©ÎïÊÇС·Ö×ÓÎïÖÊ£¬Ö»ÓÐÃâÒß·´Ó¦ÐÔ£¬Ã»ÓÐÃâÒßÔÐÔ£¬²»ÄÜÓÕ·¢»úÌå²úÉúÃâÒßÓ¦´ð£¬±ØÐëÓë´ó·Ö×ÓÔØÌåµ°°×żÁªºó²Å¾ßÓÐÃâÒßÔÐÔ¡£a.ÃâÒßÔµÄÖÆ±¸-Í·æßÀàÒ©ÎïÓëÂÑÇå°×µ°°×żÁªÎïºÏ³Éȡͷæß¾úËØC16mg¼ÓÈëIml ¶þ¼×»ù¼×õ£°·(DMF)ÈܽâµÃµ½ÈÜÒºI£¬È¡30. 5mgµÄ EDCÓÃO. 5mlµÄË®ÍêÈ«Èܽâºó£¬ÔÚ½Á°èÌõ¼þϼÓÈëµ½ÈÜÒºIÖУ¬ÊÒη´Ó¦24hµÃµ½ÈÜÒºII £» È¡40mgţѪÇå°×µ°°×(BSA)Óë5ml O. 01mmol/L pH7. 4µÄÁ×ËáÑλº³åÒº(PBS)»ìºÏ£¬Õñµ´Èܽ⣬µÃµ½ÈÜÒºIII ;ÔÚ´ÅÁ¦½Á°èÏ£¬½«ÈÜÒºIIÖðµÎ¼ÓÈëµ½ÈÜÒºIIIÖУ¬Ãܷ⣬ÊÒÎÂϽÁ°è·´Ó¦6h µÃµ½·´Ó¦ÒºIV¼´Îª¦Â-ÄÚõ£°·ÀàÒ©ÎïÃâÒßÔ£»½«·´Ó¦ÒºIV×°Èë͸Îö´üÖУ¬4¡ãC͸ÎöÈýÌ죬·Ö×°£¬ÓÚ-20¡ãC±£´æ±¸Óá£b.°ü±»ÔµÄÖÆ±¸-Í·æßÀàÒ©ÎïÓëţѪÇå°×µ°°×żÁªÎïºÏ³Éȡͷæß¾úËØC12mg¼ÓÈëIml ¶þ¼×»ù¼×õ£°·(DMF)ÈܽâµÃµ½ÈÜÒºI£¬È¡24mgµÄEDCÓÃ
O.5mlµÄË®ÍêÈ«Èܽâºó£¬ÔÚ½Á°èÌõ¼þϼÓÈëµ½ÈÜÒºIÖУ¬ÊÒη´Ó¦24hµÃµ½ÈÜÒºII ;È¡40mg ÂÑÇå°×µ°°×(OVA)Óë5ml O. 01mmol/L pH7. 4µÄÁ×ËáÑλº³åÒº(PBS)»ìºÏ£¬Õñµ´Èܽ⣬µÃµ½ÈÜÒºIII ;ÔÚ´ÅÁ¦½Á°èÏ£¬½«ÈÜÒºIIÖðµÎ¼ÓÈëµ½ÈÜÒºIIIÖУ¬Ãܷ⣬ÊÒÎÂϽÁ°è·´Ó¦6hµÃµ½·´Ó¦Òº IV¼´Îª¦Â-ÄÚõ£°·ÀàÒ©Îï°ü±»Ô£»½«·´Ó¦ÒºIV×°Èë͸Îö´üÖУ¬4¡ãC͸ÎöÈýÌ죬·Ö×°£¬ÓÚ-20¡ãC ±£´æ±¸Óá£c.Í·æßÀàÒ©Îï-ÔØÌåżÁªÎïµÄ¼ø¶¨[0050]½«ÔØÌåµ°°×¡¢Í·æßÀàÒ©ÎͷæßÀàÒ©Îï-ÔØÌåµ°°×żÁªÎïÓÃPH7.4µÄPBSÅä³ÉO. 5mg/mlµÄÈÜÒº£¬ÒÔO. 01mol/L ¦Ñ¦§7¡¤ 4 PBSµ÷Á㣬ÓÃ×ÏÍâ·Ö¹â¹â¶È¼ÆÔÚ²¨³¤200-800 nm·¶Î§ÄÚɨÃ裬µÃµ½ÔØÌåµ°°×¡¢Í·æßÀàÒ©ÎͷæßÀàÒ©Îï-ÔØÌåµ°°×żÁªÎïµÄÎüÊÕÇúÏß¡£ÈýÕß³öÏÖ²»Í¬µÄÎüÊÕÇúÏߣ¬±íÃ÷Í·æßÀàÒ©ÎïÓëÔØÌåµ°°×żÁª³É¹¦¡£2)¡¢Èý¾ÛÇè°·°ë¿¹Ô-ÔØÌåµ°°×żÁªÎïµÄºÏ³ÉÓë¼ø¶¨a.Èý¾ÛÇè°·°ë¿¹ÔºÏ³ÉÈ¡O. 64gÈý¾ÛÇèËá¶þõ£°·ºÍI. OgçúçêËáôû£¬¼ÓÈëIOmlßÁण¬ÊÒÎÂϽÁ°è12_24h£¬³ýÈ¥ÈܼÁºóÔÚÒÒ´¼-Ë®ÖÐÖØ½á¾§µÃµ½°×É«·Ûĩ״¾§Ì壬ΪÈý¾ÛÇèËá°·µÄ°ëçúçêËáõ¥£¬¼´ÎªÈý¾ÛÇè°·°ë¿¹Ô¡£b.ÃâÒßÔµÄÖÆ±¸-Èý¾ÛÇè°·ÓëBSAżÁªÎïºÏ³ÉÈ¡20mgÈý¾ÛÇè°·°ë¿¹Ô£¬ÈܽâÓÚI. Oml DMFÖеõ½ÈÜÒºI£¬ÔÙÈ¡15mgµÄEDCÓÃ
O.ImË®³ä·ÖÈܽâºó¼ÓÈëµ½ÈÜÒºIÖУ¬ÊÒνÁ°è24h£¬¼´¿ÉµÃµ½ÈÜÒºII ;³ÆÈ¡BSA30mg£¬Ê¹Ö®³ä·ÖÈܽâÔÚ7. 8ml pHΪ7. 2µÄPBSÖУ¬½«ÈÜÒºIIÖðµÎ»ºÂýµÎ¼Óµ½ÉÏÊöBSAÈÜÒºÖУ¬ÓÚÊÒÎÂϽÁ°è24hµÃµ½ÈÜÒºIII ;ÓÃO. 01mol/L PBSÔÚ4¡ãCÏÂ͸Îö3Ì죬ÒÔ³ýȥδ·´Ó¦µÄС·Ö×ÓÎïÖÊ¡£½«µÃµ½µÄÃâÒßÔ·Ö×°£¬ÓÚ_20¡ãC±£´æ±¸Óá£c.°ü±»ÔµÄÖÆ±¸-Èý¾ÛÇè°·ÓëOVAżÁªÎïºÏ³ÉÈ¡20mgÈý¾ÛÇè°·°ë¿¹Ô£¬ÈܽâÓÚI. Oml DMFÖеõ½ÈÜÒºI£¬ÔÙÈ¡15mgµÄEDCÓÃ
O.ImlË®³ä·ÖÈܽâºó¼ÓÈëµ½ÈÜÒºIÖУ¬ÊÒνÁ°è24h£¬¼´µÃÈÜÒºII ;³ÆÈ¡0VA40mg£¬Ê¹Ö®³ä·ÖÈܽâÔÚ4. 8ml pHΪ7. 2µÄPBSÖУ¬½«ÈÜÒºIIÖðµÎ»ºÂýµÎ¼Óµ½ÉÏÊöOVAÈÜÒºÖУ¬²¢ÓÚÊÒÎÂϽÁ°è24hµÃµ½ÈÜÒºIII ;ÓÃO. 01mol/LµÄPBSÔÚ4¡ãCÏÂ͸Îö3Ì죬ÒÔ³ýȥδ·´Ó¦µÄС·Ö×ÓÎïÖÊ£»½«µÃµ½µÄ°ü±»Ô·Ö×°£¬ÓÚ_20¡ãC±£´æ±¸Óá£d¡¢Èý¾ÛÇè°·-ÔØÌåżÁªÎïµÄ¼ø¶¨½«ÔØÌåµ°°×¡¢Èý¾ÛÇè°·¡¢Èý¾ÛÇè°·°ë¿¹Ô-ÔØÌåµ°°×żÁªÎïÓÃpH7. 4µÄPBSÅä³É0. 5mg/mlµÄÈÜÒº£¬ÒÔ0. 01mol/L ¦Ñ¦§7¡¤ 4 PBSµ÷Á㣬ÓÃ×ÏÍâ·Ö¹â¹â¶È¼ÆÔÚ²¨³¤200-800 nm·¶Î§ÄÚɨÃ裬µÃµ½ÔØÌåµ°°×¡¢Èý¾ÛÇè°·¡¢Èý¾ÛÇè°·°ë¿¹Ô-ÔØÌåµ°°×żÁªÎïµÄÎüÊÕÇúÏß¡£ÈýÕß³öÏÖ²»Í¬µÄÎüÊÕÇúÏߣ¬±íÃ÷Èý¾ÛÇè°·°ë¿¹ÔÓëÔØÌåµ°°×żÁª³É¹¦¡£2¡¢Í·æßÀàÒ©ÎïºÍÈý¾ÛÇè°·µÄµ¥¿Ë¡¿¹ÌåµÄÖÆ±¸(I)ÖÆ±¸µ¥¿¹a.¶¯ÎïÃâÒß½«²½ÖèIµÃµ½µÄÁ½ÖÖÃâÒßÔ·Ö±ð×¢Èëµ½Balb/cСÊóÌåÄÚ£¬ÃâÒß¼ÁÁ¿Îª15(^g/Ö»£¬Ê¹Æä²úÉú¿¹ÑªÇå¡£b.ϸ°ûÈںϺͿË¡»¯È¡ÃâÒßBalb/cСÊóƢϸ°û£¬°´9 :1(ÊýÁ¿Åä±È)±ÈÀýÓëSP2/0¹ÇËèÁöϸ°ûÈںϣ¬É¸Ñ¡µÃµ½Îȶ¨·ÖÃÚÍ·æßÀàÒ©Îïµ¥¿Ë¡¿¹ÌåµÄÍ·æßÀàÒ©Îïµ¥¿Ë¡ÔÓ½»Áöϸ°ûÖêºÍÎȶ¨·ÖÃÚÈý¾ÛÇè°·µ¥¿Ë¡¿¹ÌåµÄÈý¾ÛÇè°·µ¥¿Ë¡ÔÓ½»Áöϸ°ûÖê¡£c.ϸ°û¶³´æºÍ¸´ËÕ½«ÔÓ½»Áöϸ°ûÓö³´æÒºÖƳÉIX IO9¸ö/mlµÄϸ°ûÐüÒº£¬ÔÚÒºµªÖг¤ÆÚ±£´æ¡£¸´ËÕʱȡ³ö¶³´æ¹Ü£¬Á¢¼´·ÅÈë37 ¡ãCˮԡÖÐËÙÈÚ£¬ÀëÐÄÈ¥³ý¶³´æÒººó£¬ÒÆÈëÅàÑøÆ¿ÄÚÅàÑø¡£d.µ¥¿Ë¡¿¹ÌåµÄÖÆ±¸Óë´¿»¯[0069]ÔöÁ¿ÅàÑø·¨½«ÔÓ½»Áöϸ°ûÖÃÓÚϸ°ûÅàÑø»ùÖУ¬ÔÚ37¡ãCÌõ¼þϽøÐÐÅàÑø£¬ÓÃÐÁËá-±¥ºÍÁòËáï§·¨½«µÃµ½µÄÅàÑøÒº½øÐд¿»¯£¬µÃµ½µ¥¿Ë¡¿¹Ì壬_20¡ãC±£´æ¡£ËùÊöϸ°ûÅàÑø»ùΪÏòRPMI-1640ÅàÑø»ùÖÐÌí¼ÓСţѪÇåºÍ̼ËáÇâÄÆ£¬Ê¹Ð¡Å£ÑªÇåÔÚϸ°ûÅàÑø»ùÖеÄÖÕŨ¶ÈΪ20% (ÖÊÁ¿°Ù·Öº¬Á¿)£¬Ê¹Ì¼ËáÇâÄÆÔÚϸ°ûÅàÑø»ùÖеÄÖÕŨ¶ÈΪ
O.2% (ÖÊÁ¿°Ù·Öº¬Á¿)£»ËùÊöϸ°ûÅàÑø»ùµÄpHΪ7. 4¡£3¡¢Ñò¿¹Ê󿹿¹ÌåµÄÖÆ±¸ÒÔÑò×÷ΪÃâÒß¶¯ÎÒÔÊóÔ´¿¹ÌåΪÃâÒßÔ¶ÔÎÞ²¡ÔÌåÑò½øÐÐÃâÒߣ¬µÃµ½Ñò¿¹Ê󿹿¹Ìå¡£4¡¢µ¥¿Ë¡¿¹Ìå-½ºÌå½ð±ê¼ÇÎïµÄÖÆ±¸(I)½ºÌå½ðµÄÖÆ±¸ÓÃË«ÕôÈ¥Àë×ÓË®½«1%ÂȽðËá(¹ºÓÚsigma¹«Ë¾£¬²úƷĿ¼ºÅT09041)Ï¡ÊͳÉO. 01% (ÖÊÁ¿°Ù·Öº¬Á¿)£¬ÖôÅÁ¦¼ÓÈȰô½Á°èÆ÷ÉϽÁ°èÖó·Ð£¬Ã¿IOOml O. 01%ÂȽðËá¼ÓÈë2. 5 ml 1% ÄûÃÊËáÈýÄÆ(¹ºÓÚ¹ãÖÝ»¯Ñ§ÊÔ¼Á³§£¬²úƷĿ¼ºÅBG11-AR-01KG)£¬¼ÌÐø½Á°è¼ÓÈÈ·´Ó¦ÖÁÒºÌå³Êºìɫʱֹͣ¼ÓÈÈ£¬ÀäÈ´ÖÁÊÒκó²¹×ãʧˮ¡£ÖƱ¸ºÃµÄ½ºÌå½ðÍâ¹Û´¿¾»¡¢Í¸ÁÁ¡¢ÎÞ³ÁµíºÍƯ¸¡Îï¡£(2)µ¥¿Ë¡¿¹Ìå-½ºÌå½ð±ê¼ÇÎïµÄÖÆ±¸ÔÚ´ÅÁ¦½Á°èÏÂ,ÓÃO. 2mol/L̼Ëá¼Øµ÷½ºÌå½ðµÄpHÖµÖÁ7. O,°´50-100 ¦Ì g¿¹Ìå/ ml½ºÌå½ðµÄ±ê×¼Ïò½ºÌå½ðÈÜÒºÖмÓÈëÉÏÊöÍ·æßÀàÒ©Îïµ¥¿Ë¡¿¹ÌåºÍÈý¾ÛÇè°·µ¥¿Ë¡¿¹Ì壬 ¼ÌÐø½Á°è»ìÔÈ30min£¬¼ÓÈë15%BSAÖÁBSAÔÚ½ºÌå½ðÈÜÒºÖеÄÖÕŨ¶ÈΪ1%(Ìå»ý°Ù·Öº¬Á¿)£¬ ¾²ÖÃ30min¡£12000rpm¡¢4¡ãCÀëÐÄ30min£¬ÆúÉÏÇåÒº£¬³ÁµíÓø´ÈÜ»º³åҺϴµÓÁ½´Î£¬ÓÃÌå»ýΪ³õʼ½ºÌå½ðÌå»ý1/20µÄ¸´ÈÜ»º³åÒº½«³ÁµíÖØÐü£¬µÃµ½µÄÍ·æßÀàÒ©Îïµ¥¿Ë¡¿¹Ìå-½ºÌå½ð±ê¼ÇÎïÈÜÒººÍÈý¾ÛÇè°·µ¥¿Ë¡¿¹Ìå-½ºÌå½ð±ê¼ÇÎïÈÜÒºµÄŨ¶È·Ö±ðΪ50 ¦Ì gµ¥¿¹/mlÈÜÒº£¬ ÖÃ4¡ãC±¸Ó᣸´ÈÜ»º³åÒºº¬ÀÒµ°°×¡¢ÍÂÎÂ-80µÄO. 02mol/L¡¢pH7. 2µÄÁ×ËáÑÎÈÜÒº£¬ÆäÖÐÀÒµ°°×ÔÚ¸´ÈÜ»º³åÒºÖеÄÖÕŨ¶ÈΪO. 05%-0. 1% (Ìå»ý°Ù·Öº¬Á¿)£¬ÍÂÎÂ-80ÔÚ¸´ÈÜ»º³åÒºÖеÄÖÕŨ¶ÈΪO. 05%-0. 15% (ÖÊÁ¿°Ù·Öº¬Á¿)¡£5¡¢µ¥¿Ë¡¿¹Ìå-½ºÌå½ð±ê¼ÇÎï΢¿×ÊÔ¼Á¶³¸Éµ½Î¢¿×ÌõµÄ΢¿×ÖÐÏò΢¿×ÌõµÄ΢¿×ÖмÓÈë50 ¦Ì IÍ·æßÀàÒ©Îïµ¥¿Ë¡¿¹Ìå-½ºÌå½ð±ê¼ÇÎïºÍ50 ¦Ì IÈý¾ÛÇè°·µ¥¿Ë¡¿¹Ìå-½ºÌå½ð±ê¼ÇÎ·ÅÈëÀä¶³¸ÉÔï»úÖУ¬ÀäÚåζÈΪ_70¡ãCÌõ¼þÏ£¬Ô¤¶³4h ºó£¬ÔÙ¶³¸É14h£¬¼´¿ÉÈ¡³ö£¬µÃµ½µÄ΢¿×ÌõµÄ΢¿×Öж³¸ÉÓÐÍ·æßÀàÒ©Îïµ¥¿Ë¡¿¹Ìå-½ºÌå½ð±ê¼ÇÎïÈý¾ÛÇè°·µ¥¿Ë¡¿¹Ìå-½ºÌå½ð±ê¼ÇÎïµÄ΢¿×ÊÔ¼Á¡£6¡¢ÑùÆ·ÎüÊÕµæµÄ×¼±¸½«ÑùÆ·ÎüÊÕµæÖÃÓÚº¬BSA (BSAÔÚ»º³åÒºÖеÄÖÕŨ¶ÈΪO. 5% (Ìå»ý°Ù·Öº¬Á¿))¡¢ pHΪ7. 2¡¢0. lmol/LÁ×ËáÑλº³åÒº½þÅÝ2h£¬37¡ãCºæ2h±¸Óá£7¡¢·´Ó¦Ä¤µÄÖÆ±¸°ü±»¹ý³ÌÓÃÁ×ËỺ³åÒº½«Í·æßÀàÒ©Îï-ţѪÇå°×µ°°×żÁªÎïÏ¡Ê͵½10mg/mL£¬Óà BiodotµãĤÒǽ«Æä°ü±»ÓÚÏõËáÏËÎ¬ËØÄ¤Éϵļì²âÏß(B)°ü±»Á¿ÎªI. 0¦Ì g/cm2 ;ÓÃÁ×ËỺ³åÒº½«Èý¾ÛÇè°·°ë¿¹Ô-ţѪÇå°×µ°°×żÁªÎïÏ¡Ê͵½10mg/mL£¬ÓÃBiodotµãĤÒǽ«Æä°ü±»ÓÚÏõËáÏËÎ¬ËØÄ¤Éϵļì²âÏß(T)£¬°ü±»Á¿ÎªI. O ¦Ì g/cm2 ;ÓÃO. Olmol/L¡¢pH 7. 4 PBS»º³åÒº½«Ñò¿¹ÊóIgG¿¹ÌåÏ¡Ê͵½200¦Ì g/ml£¬ÓÃBiodotµãĤÒǽ«Æä°ü±»ÓÚÏõËáÏËÎ¬ËØÄ¤ÉϵÄÖÊ ¿ØÏß
(C)£¬°ü±»Á¿Îªl.Oyg/cm2¡£½«°ü±»ºÃµÄ·´Ó¦Ä¤ÖÃÓÚ37¡ãCÌõ¼þϸÉÔï2h£¬±¸Óá£
ȨÀûÒªÇó1.Ò»ÖÖ¼ì²â¦Â-ÄÚõ£°·À࿹ÉúËØºÍÈý¾ÛÇè°·µÄÊÔ¼ÁºÐ£¬ÆäÌØÕ÷ÔÚÓÚ°üÀ¨ÊÔÖ½Ìõ¡¢Î¢¿×Ìõ¡¢Î¢¿×ÊÔ¼ÁºÍ΢¿×Èû£¬ËùÊöÊÔÖ½Ìõ°üÀ¨·´Ó¦Ä¤¡¢ÑùÆ·ÎüÊյ桢ÎüË®µæ¡¢±£»¤Ä¤¡¢µ×°å£¬ËùÊö·´Ó¦Ä¤ÉÏÓÐÁ½Ìõ¼ì²âÏߺÍÒ»ÌõÖÊ¿ØÏߣ¬Á½Ìõ¼ì²âÏß·Ö±ð°ü±»ÓÐÍ·æßÀàÒ©Îï-ÔØÌåµ°°×żÁªÎïºÍÈý¾ÛÇè°·°ë¿¹Ô-ÔØÌåµ°°×żÁªÎÖÊ¿ØÏß°ü±»Óп¹¿¹Ì壬ËùÊö΢¿×ÊÔ¼ÁΪ¶³¸ÉµÄÍ·æßÀàÒ©ÎïÌØÒìÐÔ¿¹Ìå-½ºÌå½ð±ê¼ÇÎïºÍÈý¾ÛÇè°·ÌØÒìÐÔ¿¹Ìå-½ºÌå½ð±ê¼ÇÎï¡£
2.¸ù¾ÝȨÀûÒªÇóIËùÊöµÄÊÔ¼ÁºÐ£¬ÆäÌØÕ÷ÔÚÓÚËùÊö·´Ó¦Ä¤ÉϵÄÁ½Ìõ¼ì²âÏߺÍÒ»ÌõÖÊ¿ØÏß»¥ÏàÆ½ÐУ¬²¢ÇÒÓëÊÔÖ½ÌõµÄ³¤Ïà´¹Ö±¡£
3.¸ù¾ÝȨÀûÒªÇóIËùÊöµÄÊÔ¼ÁºÐ£¬ÆäÌØÕ÷ÔÚÓÚËùÊöµÄÈý¾ÛÇè°·¼ì²âÏß¿¿½üÑùÆ·ÎüÊÕµæÒ»¶Ë£¬ÖÊ¿ØÏß¿¿½üÎüË®µæÒ»¶Ë£¬¦Â_ÄÚõ£°·ÀàÒ©Îï¼ì²âÏßÔÚÈý¾ÛÇè°·¼ì²âÏߺÍÖÊ¿ØÏßÖ®¼ä¡£
4.¸ù¾ÝȨÀûÒªÇóIËùÊöµÄÊÔ¼ÁºÐ£¬ÆäÌØÕ÷ÔÚÓÚËùÊöÊÔÖ½ÓÉÑùÆ·ÎüÊյ桢·´Ó¦Ä¤¡¢ÎüË®µæ¡¢±£»¤Ä¤ÒÀ´Îð¤ÌùÔÚµ×°åÉÏ×é³É¡£
5.¸ù¾ÝȨÀûÒªÇóI»ò4ËùÊöµÄÊÔ¼ÁºÐ£¬ÆäÌØÕ÷ÔÚÓÚËùÊö±£»¤Ä¤Õ³ÌùÑùÆ·ÎüÊÕµæÉϵļì²â¶Ë£¬ÉÏÃæÓÐMAX±ê¼ÇÏß¡£
6.¸ù¾ÝȨÀûÒªÇóIËùÊöÊÔ¼ÁºÐ£¬ÆäÌØÕ÷ÔÚÓÚËùÊö΢¿×ÌõÉϾßÓÐ΢¿×Èû¡£
7.¸ù¾ÝȨÀûÒªÇóIËùÊöÊÔ¼ÁºÐ£¬ÆäÌØÕ÷ÔÚÓÚËùÊöÊÔ¼ÁºÐ»¹°üÀ¨ÓÃÓÚÊ¢×°ÊÔÖ½ÌõºÍ¶³¸ÉÓÐÍ·æßÀàÒ©ÎïÌØÒìÐÔ¿¹Ìå-½ºÌå½ð±ê¼ÇÎïºÍÈý¾ÛÇè°·ÌØÒìÐÔ¿¹Ìå-½ºÌå½ð±ê¼ÇÎïµÄ΢¿×ÌõµÄÊÔ¼ÁͰºÍ¹Ì¶¨ÊÔ¼ÁͰµÄ¹Ì¶¨Óþߡ£
8.¸ù¾ÝȨÀûÒªÇó7ËùÊöµÄÊÔ¼ÁºÐ£¬ÆäÌØÕ÷ÔÚÓÚËùÊöÊÔ¼ÁͰÉϾßÓÐÃÜ·â¸Ç¡£
9.¸ù¾ÝȨÀûÒªÇó7ËùÊöÊÔ¼ÁºÐ£¬ÆäÌØÕ÷ÔÚÓÚËùÊöÊÔ¼ÁºÐºÐÌåΪӲֽºÐ£¬ÊÔ¼ÁͰΪËÜÁÏÊÔ¼ÁͰ£¬¹Ì¶¨ÓþßΪӲÖÊÖ§³Å²ÄÁÏ¡£
רÀûÕªÒª±¾ÊµÓÃÐÂÐ͹«¿ªÁËÒ»ÖÖ¼ì²â¦Â-ÄÚõ£°·À࿹ÉúËØºÍÈý¾ÛÇè°·µÄÊÔ¼ÁºÐ¡£ÊÔ¼ÁºÐ°üÀ¨Î¢¿×Ìõ¡¢Î¢¿×ÊÔ¼Á¡¢ÊÔÖ½ÌõºÍ΢¿×Èû£¬ËùÊöÊÔÖ½ÌõÓÉÑùÆ·ÎüÊյ桢·´Ó¦Ä¤¡¢ÎüË®µæ¡¢±£»¤Ä¤¡¢µ×°åÒÀ´ÎÁ¬½Ó×é³É£¬ËùÊö·´Ó¦Ä¤ÉϰüÀ¨Á½Ìõ¼ì²âÏߺÍÒ»ÌõÖÊ¿ØÏߣ¬Á½Ìõ¼ì²âÏß·Ö±ð°ü±»ÓÐÍ·æßÀàÒ©Îï-ÔØÌåµ°°×żÁªÎïºÍÈý¾ÛÇè°·°ë¿¹Ô-ÔØÌåµ°°×żÁªÎÖÊ¿ØÏß°ü±»ÓÐÑò¿¹Ê󿹿¹Ìå¡£Óñ¾ÊµÓÃÐÂÐÍÊÔ¼ÁºÐͬʱ¼ì²â¦Â-ÄÚõ£°·À࿹ÉúËØºÍÈý¾ÛÇè°·µÄ·½·¨£¬¼ò±ã¡¢¿ìËÙ¡¢Ö±¹Û¡¢×¼È·¡¢±ãÓÚЯ´ø¡¢ÊÊÓ÷¶Î§¹ã¡¢³É±¾µÍ¡¢Ò×ÍÆ¹ãʹÓá£
Îĵµ±àºÅG01N33/577GK202486141SQ20112044550
¹«¿ªÈÕ2012Äê10ÔÂ10ÈÕ ÉêÇëÈÕÆÚ2011Äê11ÔÂ11ÈÕ ÓÅÏÈȨÈÕ2011Äê11ÔÂ11ÈÕ
·¢Ã÷ÕßÍòÓîÆ½, ºÎ·½Ñó, ·ë¾², ÎâÅô, ´ÞÑ廢, ´Þº£·å, ¶ÎÓ¯Ó¯, ÂÞÏþÇÙ, Äôö©Ó¨, ¹ùÐñ ÉêÇëÈË:±±¾©ÇÚ°îÉúÎï¼¼ÊõÓÐÏÞ¹«Ë¾